ID   RFAH_ECOL5              Reviewed;         162 AA.
AC   Q0TAL4;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Transcription antitermination protein RfaH {ECO:0000255|HAMAP-Rule:MF_00951};
GN   Name=rfaH {ECO:0000255|HAMAP-Rule:MF_00951}; OrderedLocusNames=ECP_4055;
OS   Escherichia coli O6:K15:H31 (strain 536 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=362663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=536 / UPEC;
RX   PubMed=16879640; DOI=10.1111/j.1365-2958.2006.05255.x;
RA   Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U.,
RA   Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.;
RT   "Role of pathogenicity island-associated integrases in the genome
RT   plasticity of uropathogenic Escherichia coli strain 536.";
RL   Mol. Microbiol. 61:584-595(2006).
RN   [2]
RP   FUNCTION IN BIOFILM FORMATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=536 / UPEC;
RX   PubMed=16452414; DOI=10.1128/jb.188.4.1316-1331.2006;
RA   Beloin C., Michaelis K., Lindner K., Landini P., Hacker J., Ghigo J.M.,
RA   Dobrindt U.;
RT   "The transcriptional antiterminator RfaH represses biofilm formation in
RT   Escherichia coli.";
RL   J. Bacteriol. 188:1316-1331(2006).
CC   -!- FUNCTION: Enhances distal genes transcription elongation in a
CC       specialized subset of operons that encode extracytoplasmic components.
CC       RfaH is recruited into a multi-component RNA polymerase complex by the
CC       ops element, which is a short conserved DNA sequence located downstream
CC       of the main promoter of these operons. Once bound, RfaH suppresses
CC       pausing and inhibits Rho-dependent and intrinsic termination at a
CC       subset of sites. Termination signals are bypassed, which allows
CC       complete synthesis of long RNA chains (By similarity). Also negatively
CC       controls expression and surface presentation of AG43 and possibly
CC       another AG43-independent factor that mediates cell-cell interactions
CC       and biofilm formation,. {ECO:0000255|HAMAP-Rule:MF_00951,
CC       ECO:0000269|PubMed:16452414}.
CC   -!- SUBUNIT: Interacts with both the nontemplate DNA and the RNA polymerase
CC       (RNAP). {ECO:0000255|HAMAP-Rule:MF_00951}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation results in increased initial
CC       adhesion and biofilm formation. {ECO:0000269|PubMed:16452414}.
CC   -!- SIMILARITY: Belongs to the RfaH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00951}.
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DR   EMBL; CP000247; ABG72015.1; -; Genomic_DNA.
DR   RefSeq; WP_001192400.1; NC_008253.1.
DR   AlphaFoldDB; Q0TAL4; -.
DR   BMRB; Q0TAL4; -.
DR   SMR; Q0TAL4; -.
DR   STRING; 362663.ECP_4055; -.
DR   EnsemblBacteria; ABG72015; ABG72015; ECP_4055.
DR   KEGG; ecp:ECP_4055; -.
DR   HOGENOM; CLU_067287_5_0_6; -.
DR   OMA; NQGVECF; -.
DR   Proteomes; UP000009182; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0001073; F:transcription antitermination factor activity, DNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00951; RfaH; 1.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR010215; Transcription_antiterm_RfaH.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   Pfam; PF02357; NusG; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR01955; RfaH; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Transcription; Transcription antitermination;
KW   Transcription regulation.
FT   CHAIN           1..162
FT                   /note="Transcription antitermination protein RfaH"
FT                   /id="PRO_0000422181"
SQ   SEQUENCE   162 AA;  18356 MW;  2A3B9B47EBB59385 CRC64;
     MQSWYLLYCK RGQLQRAQEH LERQAVNCLA PMITLEKIVR GKRTAVSEPL FPNYLFVEFD
     PEVIHTTTIN ATRGVSHFVR FGASPAIVPS AVIHQLSVYK PKDIVDPSTP YPGDKVIITE
     GAFEGFQAIF TEPDGEARSM LLLNLINKEI KHSVKNTEFR KL