ID   RFAH_ECOL6              Reviewed;         162 AA.
AC   Q8FBI4;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Transcription antitermination protein RfaH {ECO:0000255|HAMAP-Rule:MF_00951};
GN   Name=rfaH {ECO:0000255|HAMAP-Rule:MF_00951}; OrderedLocusNames=c4789;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   FUNCTION IN VIRULENCE.
RC   STRAIN=O6:K5:H1 / Nissle 1917;
RX   PubMed=15727837; DOI=10.1016/j.femsle.2005.01.038;
RA   Nagy G., Dobrindt U., Grozdanov L., Hacker J., Emody L.;
RT   "Transcriptional regulation through RfaH contributes to intestinal
RT   colonization by Escherichia coli.";
RL   FEMS Microbiol. Lett. 244:173-180(2005).
CC   -!- FUNCTION: Enhances distal genes transcription elongation in a
CC       specialized subset of operons that encode extracytoplasmic components.
CC       RfaH is recruited into a multi-component RNA polymerase complex by the
CC       ops element, which is a short conserved DNA sequence located downstream
CC       of the main promoter of these operons. Once bound, RfaH suppresses
CC       pausing and inhibits Rho-dependent and intrinsic termination at a
CC       subset of sites. Termination signals are bypassed, which allows
CC       complete synthesis of long RNA chains (By similarity). Contributes to
CC       intestinal colonization via regulation of the lipopolysaccharide core
CC       synthesis. {ECO:0000255|HAMAP-Rule:MF_00951,
CC       ECO:0000269|PubMed:15727837}.
CC   -!- SUBUNIT: Interacts with both the nontemplate DNA and the RNA polymerase
CC       (RNAP). {ECO:0000255|HAMAP-Rule:MF_00951}.
CC   -!- SIMILARITY: Belongs to the RfaH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00951}.
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DR   EMBL; AE014075; AAN83222.1; -; Genomic_DNA.
DR   RefSeq; WP_001192400.1; NC_004431.1.
DR   AlphaFoldDB; Q8FBI4; -.
DR   BMRB; Q8FBI4; -.
DR   SMR; Q8FBI4; -.
DR   STRING; 199310.c4789; -.
DR   EnsemblBacteria; AAN83222; AAN83222; c4789.
DR   KEGG; ecc:c4789; -.
DR   eggNOG; COG0250; Bacteria.
DR   HOGENOM; CLU_067287_5_0_6; -.
DR   OMA; NQGVECF; -.
DR   BioCyc; ECOL199310:C4789-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0001073; F:transcription antitermination factor activity, DNA binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.940; -; 1.
DR   HAMAP; MF_00951; RfaH; 1.
DR   InterPro; IPR006645; NGN_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR010215; Transcription_antiterm_RfaH.
DR   PANTHER; PTHR30265; PTHR30265; 1.
DR   Pfam; PF02357; NusG; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF82679; SSF82679; 1.
DR   TIGRFAMs; TIGR01955; RfaH; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Transcription; Transcription antitermination;
KW   Transcription regulation.
FT   CHAIN           1..162
FT                   /note="Transcription antitermination protein RfaH"
FT                   /id="PRO_0000422182"
SQ   SEQUENCE   162 AA;  18356 MW;  2A3B9B47EBB59385 CRC64;
     MQSWYLLYCK RGQLQRAQEH LERQAVNCLA PMITLEKIVR GKRTAVSEPL FPNYLFVEFD
     PEVIHTTTIN ATRGVSHFVR FGASPAIVPS AVIHQLSVYK PKDIVDPSTP YPGDKVIITE
     GAFEGFQAIF TEPDGEARSM LLLNLINKEI KHSVKNTEFR KL