RFAI_ECOLI
ID RFAI_ECOLI Reviewed; 339 AA.
AC P27128; Q2M7U1;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Lipopolysaccharide 1,3-galactosyltransferase;
DE EC=2.4.1.44;
DE AltName: Full=Lipopolysaccharide 3-alpha-galactosyltransferase;
GN Name=rfaI; Synonyms=waaI; OrderedLocusNames=b3627, JW3602;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1624461; DOI=10.1128/jb.174.14.4736-4745.1992;
RA Pradel E., Parker C.T., Schnaitman C.A.;
RT "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-
RT 12 and their roles in assembly of the lipopolysaccharide core.";
RL J. Bacteriol. 174:4736-4745(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-alpha-D-galactose + [lipopolysaccharide] = UDP + 3-alpha-
CC D-galactosyl-[lipopolysaccharide].; EC=2.4.1.44;
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
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DR EMBL; M80599; AAA24086.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18604.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76651.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77665.1; -; Genomic_DNA.
DR PIR; C42982; C42982.
DR RefSeq; NP_418084.1; NC_000913.3.
DR RefSeq; WP_001188013.1; NZ_CP047127.1.
DR AlphaFoldDB; P27128; -.
DR SMR; P27128; -.
DR BioGRID; 4263412; 188.
DR DIP; DIP-10670N; -.
DR IntAct; P27128; 3.
DR STRING; 511145.b3627; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR jPOST; P27128; -.
DR PaxDb; P27128; -.
DR PRIDE; P27128; -.
DR DNASU; 948143; -.
DR EnsemblBacteria; AAC76651; AAC76651; b3627.
DR EnsemblBacteria; BAE77665; BAE77665; BAE77665.
DR GeneID; 948143; -.
DR KEGG; ecj:JW3602; -.
DR KEGG; eco:b3627; -.
DR PATRIC; fig|1411691.4.peg.3079; -.
DR EchoBASE; EB1327; -.
DR eggNOG; COG1442; Bacteria.
DR HOGENOM; CLU_050833_5_0_6; -.
DR InParanoid; P27128; -.
DR OMA; TVFLHFC; -.
DR PhylomeDB; P27128; -.
DR BioCyc; EcoCyc:EG11352-MON; -.
DR BioCyc; MetaCyc:EG11352-MON; -.
DR UniPathway; UPA00958; -.
DR PRO; PR:P27128; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0008918; F:lipopolysaccharide 3-alpha-galactosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047270; F:lipopolysaccharide glucosyltransferase II activity; IDA:EcoCyc.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IMP:EcoCyc.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR013645; Glyco_transf_8N.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR Pfam; PF08437; Glyco_transf_8C; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Lipopolysaccharide biosynthesis; Manganese;
KW Metal-binding; Reference proteome; Transferase.
FT CHAIN 1..339
FT /note="Lipopolysaccharide 1,3-galactosyltransferase"
FT /id="PRO_0000206064"
FT BINDING 34..39
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 131..132
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 131
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 133
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 265..271
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 265
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
SQ SEQUENCE 339 AA; 39423 MW; 068CA689C1E0C1E0 CRC64;
MQQVFFQETE FLNSVIDYDH KVETENLCLD IAYGTDKNFL FGCGISIASI LKYNEGSRLC
FHIFTDYFGD DDRKYFDALA LQYKTRIKIY LINGDRLRSL PSTKNWTHAI YFRFVIADYF
INKAPKVLYL DADIICQGTI EPLINFSFPD DKVAMVVTEG QADWWEKRAH SLGVAGIAKG
YFNSGFLLIN TAQWAAQQVS ARAIAMLNEP EIIKKITHPD QDVLNMLLAD KLIFADIKYN
TQFSLNYQLK ESFINPVTND TIFIHYIGPT KPWHDWAWDY PVSQAFMEAK NASPWKNTAL
LKPNNSNQLR YSAKHMLKKH RYLKGFSNYL FYFIEKIKH
