ID   RFAP_SALTY              Reviewed;         265 AA.
AC   Q06995; O68268;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Lipopolysaccharide core heptose(I) kinase RfaP;
DE            EC=2.7.1.-;
GN   Name=rfaP; Synonyms=waaP; OrderedLocusNames=STM3721;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=9535865; DOI=10.1074/jbc.273.15.8849;
RA   Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C.;
RT   "The assembly system for the lipopolysaccharide R2 core-type of Escherichia
RT   coli is a hybrid of those found in Escherichia coli K-12 and Salmonella
RT   enterica. Structure and function of the R2 WaaK and WaaL homologs.";
RL   J. Biol. Chem. 273:8849-8859(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 177-265.
RC   STRAIN=LT2;
RX   PubMed=8444813; DOI=10.1128/jb.175.5.1524-1527.1993;
RA   Klena J.D., Pradel E., Schnaitman C.A.;
RT   "The rfaS gene, which is involved in production of a rough form of
RT   lipopolysaccharide core in Escherichia coli K-12, is not present in the rfa
RT   cluster of Salmonella typhimurium LT2.";
RL   J. Bacteriol. 175:1524-1527(1993).
CC   -!- FUNCTION: Catalyzes the phosphorylation of heptose(I) of the outer
CC       membrane lipopolysaccharide core. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. KdkA/RfaP
CC       family. {ECO:0000305}.
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DR   EMBL; AF026386; AAC16414.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22580.1; -; Genomic_DNA.
DR   EMBL; S56361; AAB25550.1; -; Genomic_DNA.
DR   PIR; A47074; A47074.
DR   RefSeq; NP_462621.1; NC_003197.2.
DR   RefSeq; WP_000229825.1; NC_003197.2.
DR   AlphaFoldDB; Q06995; -.
DR   SMR; Q06995; -.
DR   STRING; 99287.STM3721; -.
DR   PaxDb; Q06995; -.
DR   DNASU; 1255245; -.
DR   EnsemblBacteria; AAL22580; AAL22580; STM3721.
DR   GeneID; 1255245; -.
DR   KEGG; stm:STM3721; -.
DR   PATRIC; fig|99287.12.peg.3935; -.
DR   HOGENOM; CLU_081267_0_0_6; -.
DR   OMA; GWGEIFK; -.
DR   PhylomeDB; Q06995; -.
DR   BioCyc; MetaCyc:STM3721-MON; -.
DR   BioCyc; SENT99287:STM3721-MON; -.
DR   UniPathway; UPA00958; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR017172; Lsacc_core_hep_kinase_RfaP.
DR   PIRSF; PIRSF037318; RfaP; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Lipopolysaccharide biosynthesis; Magnesium;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..265
FT                   /note="Lipopolysaccharide core heptose(I) kinase RfaP"
FT                   /id="PRO_0000097286"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000255"
FT   CONFLICT        224
FT                   /note="F -> Y (in Ref. 3; AAB25550)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   265 AA;  30996 MW;  9D3BF9C319667F30 CRC64;
     MVELKAPLTT LWRGKDAFEE VKTLQGEVFR ELETRRTLRF ELDGKSYFLK WHKGTSLKEI
     VKNLISLRMP VLGADREWHA IHRLHELGVD TMHGVGFGEK GVNPLTRTSF IITEDLTPTI
     SLEDYCADWA VNPPDAQVKW MIIKRVATMV RKMHAGGINH RDCYICHFLL HLPFTGREED
     LKISVIDLHR AQIRQHVPLR WRDKDLIGLY FSSMNIGLTQ RDIFRFMREY FSLPLREILQ
     KESGLIHQAD VKAARIKERT IRKNL