ID   RFAQ_ECOLI              Reviewed;         344 AA.
AC   P25742; Q2M7U6;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 2.
DT   03-AUG-2022, entry version 152.
DE   RecName: Full=Lipopolysaccharide core heptosyltransferase RfaQ;
DE            EC=2.-.-.-;
GN   Name=rfaQ; Synonyms=waaQ; OrderedLocusNames=b3632, JW3607;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1732225; DOI=10.1128/jb.174.3.930-934.1992;
RA   Parker C.T., Pradel E., Schnaitman C.A.;
RT   "Identification and sequences of the lipopolysaccharide core biosynthetic
RT   genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.";
RL   J. Bacteriol. 174:930-934(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1447141; DOI=10.1128/jb.174.23.7750-7756.1992;
RA   Clementz T.;
RT   "The gene coding for 3-deoxy-manno-octulosonic acid transferase and the
RT   rfaQ gene are transcribed from divergently arranged promoters in
RT   Escherichia coli.";
RL   J. Bacteriol. 174:7750-7756(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA   Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT   from 76.0 to 81.5 minutes.";
RL   Nucleic Acids Res. 22:2576-2586(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Catalyzes heptose transfer to the lipopolysaccharide core. It
CC       transfers a heptose, called heptose(III), to the heptose(II) of the
CC       inner core (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 9 family. {ECO:0000305}.
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DR   EMBL; M80599; AAA24081.1; -; Genomic_DNA.
DR   EMBL; M86305; AAA03744.1; -; Genomic_DNA.
DR   EMBL; U00039; AAB18609.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76656.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77660.1; -; Genomic_DNA.
DR   PIR; S27560; S27560.
DR   RefSeq; NP_418089.1; NC_000913.3.
DR   RefSeq; WP_001268851.1; NZ_JACEFS010000007.1.
DR   AlphaFoldDB; P25742; -.
DR   SMR; P25742; -.
DR   BioGRID; 4259595; 230.
DR   DIP; DIP-10675N; -.
DR   IntAct; P25742; 2.
DR   STRING; 511145.b3632; -.
DR   CAZy; GT9; Glycosyltransferase Family 9.
DR   jPOST; P25742; -.
DR   PaxDb; P25742; -.
DR   PRIDE; P25742; -.
DR   EnsemblBacteria; AAC76656; AAC76656; b3632.
DR   EnsemblBacteria; BAE77660; BAE77660; BAE77660.
DR   GeneID; 948155; -.
DR   KEGG; ecj:JW3607; -.
DR   KEGG; eco:b3632; -.
DR   PATRIC; fig|511145.12.peg.3752; -.
DR   EchoBASE; EB1317; -.
DR   eggNOG; COG0859; Bacteria.
DR   HOGENOM; CLU_038371_3_2_6; -.
DR   InParanoid; P25742; -.
DR   OMA; HMAVTQG; -.
DR   PhylomeDB; P25742; -.
DR   BioCyc; EcoCyc:EG11341-MON; -.
DR   BioCyc; MetaCyc:EG11341-MON; -.
DR   BRENDA; 2.4.99.B9; 2026.
DR   UniPathway; UPA00958; -.
DR   PRO; PR:P25742; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008713; F:ADP-heptose-lipopolysaccharide heptosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0071967; F:lipopolysaccharide core heptosyltransferase activity; IDA:EcoCyc.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IMP:EcoCyc.
DR   CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR   InterPro; IPR002201; Glyco_trans_9.
DR   InterPro; IPR011916; LipoPS_heptosylTferase-III.
DR   Pfam; PF01075; Glyco_transf_9; 1.
DR   TIGRFAMs; TIGR02201; heptsyl_trn_III; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..344
FT                   /note="Lipopolysaccharide core heptosyltransferase RfaQ"
FT                   /id="PRO_0000207265"
SQ   SEQUENCE   344 AA;  38731 MW;  1C788368BE46404A CRC64;
     MRYHGDMLLT TPVISTLKQN YPDAKIDMLL YQDTIPILSE NPEINALYGI SNKGAGTFDK
     IKNVLSLIKT LRANNYDLVI NLTDQWMVAL LVRCLPARMK ISQLYGHRQH GIWKKSFTHL
     APIHGTHIVE RNLSVLEPLG ITDFYTDTTM SYAEDCWKKM RRELDALGVK DHYVVIQPTA
     RQIFKCWDND KFSKVIDALQ QRGYQVVLTC GPSADDLACV DEIARGCETK PITGLAGKTR
     FPELGALIDH AVLFIGVDSA PGHIAAAVKT PVISLFGATD HVFWRPWTEN IIQFWAGNYQ
     KMPTRHELDR NKKYLSVIPA EDVIAATEKL LPEDAPSADR NAQL