ID   RFAQ_ECOLX              Reviewed;         340 AA.
AC   Q9R9D5;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Lipopolysaccharide core heptosyltransferase RfaQ;
DE            EC=2.-.-.-;
GN   Name=rfaQ; Synonyms=waaQ;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=F470;
RX   PubMed=9792656; DOI=10.1074/jbc.273.45.29497;
RA   Heinrichs D.E., Yethon J.A., Amor P.A., Whitfield C.;
RT   "The assembly system for the outer core portion of R1- and R4-type
RT   lipopolysaccharides of Escherichia coli. The R1 core-specific beta-
RT   glucosyltransferase provides a novel attachment site for O-
RT   polysaccharides.";
RL   J. Biol. Chem. 273:29497-29505(1998).
RN   [2]
RP   FUNCTION AS A HEPTOSYLTRANSFERASE.
RC   STRAIN=F470;
RX   PubMed=9756860; DOI=10.1074/jbc.273.41.26310;
RA   Yethon J.A., Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C.;
RT   "Involvement of waaY, waaQ, and waaP in the modification of Escherichia
RT   coli lipopolysaccharide and their role in the formation of a stable outer
RT   membrane.";
RL   J. Biol. Chem. 273:26310-26316(1998).
CC   -!- FUNCTION: Catalyzes heptose transfer to the lipopolysaccharide core. It
CC       transfers a heptose, called heptose(III), to the heptose(II) of the
CC       inner core. {ECO:0000269|PubMed:9756860}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 9 family. {ECO:0000305}.
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DR   EMBL; AF019746; AAC69679.1; -; Genomic_DNA.
DR   RefSeq; WP_001307466.1; NZ_WSIS01000023.1.
DR   AlphaFoldDB; Q9R9D5; -.
DR   SMR; Q9R9D5; -.
DR   STRING; 585034.ECIAI1_3802; -.
DR   eggNOG; COG0859; Bacteria.
DR   UniPathway; UPA00958; -.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR   InterPro; IPR002201; Glyco_trans_9.
DR   InterPro; IPR011916; LipoPS_heptosylTferase-III.
DR   Pfam; PF01075; Glyco_transf_9; 1.
DR   TIGRFAMs; TIGR02201; heptsyl_trn_III; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Transferase.
FT   CHAIN           1..340
FT                   /note="Lipopolysaccharide core heptosyltransferase RfaQ"
FT                   /id="PRO_0000383675"
SQ   SEQUENCE   340 AA;  37921 MW;  FBA620791EFA61DF CRC64;
     MRFHGDMLLT TPVISSLKKN YPDAKIDVLL YQDTIPILSE NPEINALYGI KNKKAKASEK
     IANFFHLIKV LRANKYDLIV NLTDQWMVAI LVRLLNARVK ISQDYHHRQS AFWRKSFTHL
     VPLQGGNVVE SNLSVLTPLG VDSLVKQTTM SYPPASWKRM RRELDHAGVG QNYVVIQPTA
     RQIFKCWDNA KFSAVIDALH ARGYEVVLTS GPDKDDLACV NEIAQGCQTP PVTALAGKVT
     FPELGALIDH AQLFIGVDSA PAHIAAAVNT PLISLFGATD HIFWRPWSNN MIQFWAGDYR
     EMPTRDQRDR NEMYLSVIPA ADVIAAVDKL LPSSTTGTSL