ID   RFAY_ECOLX              Reviewed;         230 AA.
AC   Q9ZIS7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Lipopolysaccharide core heptose(II) kinase RfaY;
DE            EC=2.7.1.-;
GN   Name=rfaY; Synonyms=waaY;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=F470;
RX   PubMed=9792656; DOI=10.1074/jbc.273.45.29497;
RA   Heinrichs D.E., Yethon J.A., Amor P.A., Whitfield C.;
RT   "The assembly system for the outer core portion of R1- and R4-type
RT   lipopolysaccharides of Escherichia coli. The R1 core-specific beta-
RT   glucosyltransferase provides a novel attachment site for O-
RT   polysaccharides.";
RL   J. Biol. Chem. 273:29497-29505(1998).
RN   [2]
RP   FUNCTION AS A KINASE.
RC   STRAIN=F470;
RX   PubMed=9756860; DOI=10.1074/jbc.273.41.26310;
RA   Yethon J.A., Heinrichs D.E., Monteiro M.A., Perry M.B., Whitfield C.;
RT   "Involvement of waaY, waaQ, and waaP in the modification of Escherichia
RT   coli lipopolysaccharide and their role in the formation of a stable outer
RT   membrane.";
RL   J. Biol. Chem. 273:26310-26316(1998).
CC   -!- FUNCTION: Catalyzes the phosphorylation of heptose(II) of the outer
CC       membrane lipopolysaccharide core. {ECO:0000269|PubMed:9756860}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. RfaY/WaaY
CC       family. {ECO:0000305}.
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DR   EMBL; AF019746; AAC69674.1; -; Genomic_DNA.
DR   RefSeq; WP_000633087.1; NZ_WVVT01000019.1.
DR   AlphaFoldDB; Q9ZIS7; -.
DR   STRING; 585034.ECIAI1_3797; -.
DR   eggNOG; COG0661; Bacteria.
DR   OMA; ICKANDL; -.
DR   UniPathway; UPA00958; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR009330; LipoPS_heptP_kinase.
DR   Pfam; PF06176; WaaY; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Kinase; Lipopolysaccharide biosynthesis; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..230
FT                   /note="Lipopolysaccharide core heptose(II) kinase RfaY"
FT                   /id="PRO_0000383674"
SQ   SEQUENCE   230 AA;  26994 MW;  DC54009D3B1BB098 CRC64;
     MITSIRYRGF SFYYKDNDNK YKEIFDEILA YNFKTVKVLR NIDDTKVSLI DTKYGRYVFK
     VFAPKTKRNE RFLKSFVKGD YYQNLIVETD RVRSAGLTFP NDFYFLAERK IFNYASVFIM
     LIEYVEGVEL NDMPIIPENV KAEIKASMEK LHALNMLSGD PHRGNFIVSK DGVRIIDLSG
     KSCTAERKAR DRLAMERHLG IANEIKDYGY YSVIYRTKLR KFIKKLKGKA