ID   RFBB_SINFN              Reviewed;         350 AA.
AC   P55462;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Probable dTDP-glucose 4,6-dehydratase;
DE            EC=4.2.1.46;
GN   OrderedLocusNames=NGR_a03580; ORFNames=y4gF;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC         + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC         ChEBI:CHEBI:57649; EC=4.2.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC   -!- PATHWAY: Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. dTDP-glucose dehydratase subfamily. {ECO:0000305}.
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DR   EMBL; U00090; AAB91680.1; -; Genomic_DNA.
DR   RefSeq; NP_443868.1; NC_000914.2.
DR   RefSeq; WP_010875372.1; NC_000914.2.
DR   AlphaFoldDB; P55462; -.
DR   SMR; P55462; -.
DR   STRING; 394.NGR_a03580; -.
DR   EnsemblBacteria; AAB91680; AAB91680; NGR_a03580.
DR   KEGG; rhi:NGR_a03580; -.
DR   PATRIC; fig|394.7.peg.366; -.
DR   eggNOG; COG1088; Bacteria.
DR   HOGENOM; CLU_007383_1_14_5; -.
DR   OMA; KLIPLMC; -.
DR   OrthoDB; 707977at2; -.
DR   UniPathway; UPA00124; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05246; dTDP_GD_SDR_e; 1.
DR   InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01181; dTDP_gluc_dehyt; 1.
PE   3: Inferred from homology;
KW   Lyase; NAD; Plasmid; Reference proteome.
FT   CHAIN           1..350
FT                   /note="Probable dTDP-glucose 4,6-dehydratase"
FT                   /id="PRO_0000183246"
FT   ACT_SITE        133
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        134
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        157
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         7..13
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
FT   BINDING         132
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   350 AA;  39665 MW;  B6EF2E437DD751A4 CRC64;
     MRILVTGGAG FIGSALVRYL VSINAEVLNV DKLTYAGNLA SLKPVEGLRN YRFLRADICD
     RVAINEAFET FQPDYVIHLA AESHVDRSIT GADDFVQTNV NGTFTMLETA RQYWSNLSQN
     RKAFFKMLHV STDEVYGSLG DRGQFEEVSP YDPSSPYSAS KAASDHFATA WQRTYGLPVV
     ISNCSNNYGP FHFPEKLIPL MILNALDRKP LPVYGTGSNI RDWLYVDDHA RALWLIVREG
     RPGEKYNVGG RNELRNIDVV NRICLLLDEL SPNASHYGDL ITFVKDRPGH DARYAIDATK
     LETELGWKAQ ENFDTGIRKT VEWYLENGWW WQPLRDKVYS GERLGLLEKA