ID   RFBE_SHIFL              Reviewed;         418 AA.
AC   P37781;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2003, sequence version 2.
DT   25-MAY-2022, entry version 127.
DE   RecName: Full=Putative O-antigen transporter;
GN   Name=rfbE; OrderedLocusNames=SF2100, S2222;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PE577 / Serotype 2a;
RX   PubMed=7507920; DOI=10.1128/jb.176.3.733-747.1994;
RA   Morona R., Mavris M., Fallarino A., Manning P.A.;
RT   "Characterization of the rfc region of Shigella flexneri.";
RL   J. Bacteriol. 176:733-747(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Could be an O-antigen transporter.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the polysaccharide synthase family.
CC       {ECO:0000305}.
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DR   EMBL; X71970; CAA50771.1; -; Genomic_DNA.
DR   EMBL; AE005674; AAN43639.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17468.1; -; Genomic_DNA.
DR   PIR; JC4069; JC4069.
DR   RefSeq; NP_707932.1; NC_004337.2.
DR   RefSeq; WP_000022479.1; NZ_WPGV01000030.1.
DR   AlphaFoldDB; P37781; -.
DR   SMR; P37781; -.
DR   STRING; 198214.SF2100; -.
DR   EnsemblBacteria; AAN43639; AAN43639; SF2100.
DR   EnsemblBacteria; AAP17468; AAP17468; S2222.
DR   GeneID; 1026640; -.
DR   KEGG; sfl:SF2100; -.
DR   KEGG; sfx:S2222; -.
DR   PATRIC; fig|198214.7.peg.2508; -.
DR   HOGENOM; CLU_040633_0_0_6; -.
DR   OMA; EAFGFVH; -.
DR   OrthoDB; 564091at2; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR029303; Polysacc_synt_C.
DR   InterPro; IPR002797; Polysacc_synth.
DR   Pfam; PF01943; Polysacc_synt; 1.
DR   Pfam; PF14667; Polysacc_synt_C; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..418
FT                   /note="Putative O-antigen transporter"
FT                   /id="PRO_0000166448"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        251..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        385..405
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        249
FT                   /note="D -> E (in Ref. 1; CAA50771)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        287
FT                   /note="E -> A (in Ref. 1; CAA50771)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   418 AA;  46398 MW;  DAE705311FC96008 CRC64;
     MSIIKNSVWN LFGYAIPTLI AIPSLGFLAR GLGPEGFGVY TIAIALVGYA GIFDVGLTRS
     VIREIAIHRD NHHERTKVIS TSTSFLVLFS CFGAFLLLIF SDGIVNYLKI SGVEHSDIQL
     AFKLLAICIP LFILNQLWSA ILEGDEKFGI VNIQKSISSS CIAGIPAIFV FYSATLSAAV
     AGLIFARVIS ILVSAYYVRN DIKISGVHFC YKTFKRLFFF GGWMTVSNII SPVMVYFDRF
     IVSNIMGADK VAFYSAPAEV ILKLGIIPAA IGRAVFPRLS NIKDFKEFKR NVNKSLLLMF
     LICLPVIIIG LLYSGLVLKI WFGENYQINS FNILNVLLIG FFFNALAMIP FSAIQALGKS
     KITALIHCAE LVPYLALLYF MVEKYGLLGA AISWSIRVIL DALLLQWLYT RMCSVYEN