RFC2_ARATH
ID RFC2_ARATH Reviewed; 333 AA.
AC Q9CAM7;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Replication factor C subunit 2;
DE Short=AtRFC2;
DE AltName: Full=Activator 1 subunit 2;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2811;
GN Name=RFC2; Synonyms=EMB2811; OrderedLocusNames=At1g63160;
GN ORFNames=F16M19.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: May be involved in DNA replication and thus regulate cell
CC proliferation. {ECO:0000250}.
CC -!- SUBUNIT: Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can
CC form a complex with RFC1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9CAM7; Q9CAQ8: RFC5; NbExp=3; IntAct=EBI-25513796, EBI-25513346;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family.
CC {ECO:0000305}.
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DR EMBL; AC010795; AAG51618.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34063.1; -; Genomic_DNA.
DR EMBL; BT031353; ACB88833.1; -; mRNA.
DR PIR; B96657; B96657.
DR RefSeq; NP_176504.1; NM_104994.4.
DR AlphaFoldDB; Q9CAM7; -.
DR SMR; Q9CAM7; -.
DR BioGRID; 27841; 7.
DR IntAct; Q9CAM7; 1.
DR STRING; 3702.AT1G63160.1; -.
DR iPTMnet; Q9CAM7; -.
DR PaxDb; Q9CAM7; -.
DR PRIDE; Q9CAM7; -.
DR ProteomicsDB; 236229; -.
DR EnsemblPlants; AT1G63160.1; AT1G63160.1; AT1G63160.
DR GeneID; 842620; -.
DR Gramene; AT1G63160.1; AT1G63160.1; AT1G63160.
DR KEGG; ath:AT1G63160; -.
DR Araport; AT1G63160; -.
DR TAIR; locus:2015233; AT1G63160.
DR eggNOG; KOG0991; Eukaryota.
DR HOGENOM; CLU_042324_0_1_1; -.
DR InParanoid; Q9CAM7; -.
DR OMA; SCNYSSQ; -.
DR OrthoDB; 1071197at2759; -.
DR PhylomeDB; Q9CAM7; -.
DR PRO; PR:Q9CAM7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAM7; baseline and differential.
DR Genevisible; Q9CAM7; AT.
DR GO; GO:0005663; C:DNA replication factor C complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013748; Rep_factorC_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF08542; Rep_fac_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP-binding; DNA replication; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..333
FT /note="Replication factor C subunit 2"
FT /id="PRO_0000422630"
FT BINDING 55..62
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 333 AA; 36747 MW; 055CDAD50A89976A CRC64;
MASSSSTSTG DGYNEPWVEK YRPSKVVDIV GNEDAVSRLQ VIARDGNMPN LILSGPPGTG
KTTSILALAH ELLGTNYKEA VLELNASDDR GIDVVRNKIK MFAQKKVTLP PGRHKVVILD
EADSMTSGAQ QALRRTIEIY SNSTRFALAC NTSAKIIEPI QSRCALVRFS RLSDQQILGR
LLVVVAAEKV PYVPEGLEAI IFTADGDMRQ ALNNLQATFS GFSFVNQENV FKVCDQPHPL
HVKNIVRNVL ESKFDIACDG LKQLYDLGYS PTDIITTLFR IIKNYDMAEY LKLEFMKETG
FAHMRICDGV GSYLQLCGLL AKLSIVRETA KAP
