RFC5_ARATH
ID RFC5_ARATH Reviewed; 369 AA.
AC Q9CAQ8;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Replication factor C subunit 5;
DE Short=AtRFC5;
DE AltName: Full=Activator 1 subunit 5;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2810;
GN Name=RFC5; Synonyms=EMB2810, RFC3; OrderedLocusNames=At1g77470;
GN ORFNames=T5M16.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY SALICYLIC ACID, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF GLY-84.
RX PubMed=19482917; DOI=10.1104/pp.109.138321;
RA Xia S., Zhu Z., Hao L., Chen J.G., Xiao L., Zhang Y., Li X.;
RT "Negative regulation of systemic acquired resistance by replication factor
RT C subunit3 in Arabidopsis.";
RL Plant Physiol. 150:2009-2017(2009).
RN [5]
RP FUNCTION.
RX PubMed=20023430; DOI=10.4161/psb.5.2.10526;
RA Xia S., Xiao L., Gannon P., Li X.;
RT "RFC3 regulates cell proliferation and pathogen resistance in
RT Arabidopsis.";
RL Plant Signal. Behav. 5:168-170(2010).
CC -!- FUNCTION: Functions in cell replication and proliferation. May be
CC involved in chromatin assembly and remodeling. Plays a role in the
CC negative control of pathogenesis-related gene expression and systemic
CC acquired resistance (SAR). {ECO:0000269|PubMed:19482917,
CC ECO:0000269|PubMed:20023430}.
CC -!- SUBUNIT: Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can
CC form a complex with RFC1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9CAQ8; Q9CAM7: RFC2; NbExp=3; IntAct=EBI-25513346, EBI-25513796;
CC Q9CAQ8; Q93ZX1: RFC4; NbExp=7; IntAct=EBI-25513346, EBI-4470690;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19482917}.
CC -!- INDUCTION: By salicylic acid (SA). {ECO:0000269|PubMed:19482917}.
CC -!- DISRUPTION PHENOTYPE: Moderately dwarfed plants with small organs due
CC to reduced cell proliferation rate. Enhanced resistance to pathogens.
CC {ECO:0000269|PubMed:19482917}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family.
CC {ECO:0000305}.
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DR EMBL; AC010704; AAG51681.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35982.1; -; Genomic_DNA.
DR EMBL; AY065452; AAL38893.1; -; mRNA.
DR EMBL; AY117282; AAM51357.1; -; mRNA.
DR PIR; A96804; A96804.
DR RefSeq; NP_177871.1; NM_106396.5.
DR AlphaFoldDB; Q9CAQ8; -.
DR SMR; Q9CAQ8; -.
DR BioGRID; 29302; 3.
DR IntAct; Q9CAQ8; 2.
DR STRING; 3702.AT1G77470.1; -.
DR SwissPalm; Q9CAQ8; -.
DR PaxDb; Q9CAQ8; -.
DR PRIDE; Q9CAQ8; -.
DR ProteomicsDB; 236935; -.
DR EnsemblPlants; AT1G77470.1; AT1G77470.1; AT1G77470.
DR GeneID; 844083; -.
DR Gramene; AT1G77470.1; AT1G77470.1; AT1G77470.
DR KEGG; ath:AT1G77470; -.
DR Araport; AT1G77470; -.
DR TAIR; locus:2204710; AT1G77470.
DR eggNOG; KOG0990; Eukaryota.
DR HOGENOM; CLU_042324_2_0_1; -.
DR InParanoid; Q9CAQ8; -.
DR OMA; TRTIFCG; -.
DR OrthoDB; 1071197at2759; -.
DR PhylomeDB; Q9CAQ8; -.
DR PRO; PR:Q9CAQ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAQ8; baseline and differential.
DR Genevisible; Q9CAQ8; AT.
DR GO; GO:0005663; C:DNA replication factor C complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0031348; P:negative regulation of defense response; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013748; Rep_factorC_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF08542; Rep_fac_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; DNA-binding; Nucleotide-binding; Nucleus;
KW Plant defense; Reference proteome.
FT CHAIN 1..369
FT /note="Replication factor C subunit 5"
FT /id="PRO_0000422633"
FT REGION 21..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 79..86
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 84
FT /note="G->D: In rfc3-1; reduced plant size and enhanced
FT disease resistance."
FT /evidence="ECO:0000269|PubMed:19482917"
SQ SEQUENCE 369 AA; 41370 MW; 68D21B73A535F65C CRC64;
MTELTSAMDI DVDEIQPRKP INKGKDVVGF GPPPQSKATP WVEKYRPQSL DDVAAHRDII
DTIDRLTNEN KLPHLLLYGP PGTGKTSTIL AVARKLYGPK YRNMILELNA SDDRGIDVVR
QQIQDFASTQ SFSLGKSSVK LVLLDEADAM TKDAQFALRR VIEKYTKSTR FALIGNHVNK
IIPALQSRCT RFRFAPLDGV HMSQRLKHVI EAERLVVSDC GLAALVRLSN GDMRKALNIL
QSTHMASKEI TEEESKQITE EDVYLCTGNP LPKDIEQISH WLLNKPFDEC YKDVSEIKTR
KGLAIVDIVK EITLFIFKIK MPSAVRVQLI NDLADIEYRL SFGCNDKLQL GAIISTFTHA
RSIIVGAAK
