ID   RFCL_METAC              Reviewed;         607 AA.
AC   Q8TPU4;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Replication factor C large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE            Short=RFC large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE   AltName: Full=Clamp loader large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
GN   Name=rfcL {ECO:0000255|HAMAP-Rule:MF_01508}; OrderedLocusNames=MA_1810;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC       subunits (RfcL). {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01508}.
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DR   EMBL; AE010299; AAM05216.1; -; Genomic_DNA.
DR   RefSeq; WP_011021813.1; NC_003552.1.
DR   AlphaFoldDB; Q8TPU4; -.
DR   SMR; Q8TPU4; -.
DR   STRING; 188937.MA_1810; -.
DR   PRIDE; Q8TPU4; -.
DR   EnsemblBacteria; AAM05216; AAM05216; MA_1810.
DR   GeneID; 1473699; -.
DR   KEGG; mac:MA_1810; -.
DR   HOGENOM; CLU_027255_0_0_2; -.
DR   InParanoid; Q8TPU4; -.
DR   OMA; GGWTRYG; -.
DR   OrthoDB; 24257at2157; -.
DR   PhylomeDB; Q8TPU4; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01508; RfcL; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023935; Rep_factor-C_lsu.
DR   Pfam; PF00004; AAA; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..607
FT                   /note="Replication factor C large subunit"
FT                   /id="PRO_0000135951"
FT   REGION          468..607
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        504..522
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        539..587
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        590..607
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         55..62
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01508"
SQ   SEQUENCE   607 AA;  67637 MW;  03D4B450304968B1 CRC64;
     MVWFKMMSAI EWAEKYRPRT LEDVVGNKKA VRDFRAWAEE WQSRIPETRA VILYGPAGIG
     KTSSAHALAR DMDWDVIELN ASDQRTAGVI EKIAGSAASM NTLFGSKRLI ILDEADNIHG
     TADRGGMRAI SGIIKGTLQP IVLIANDIYG LTPTIRNLCL EIKFGSVQSR SMVPALKKVC
     GAEGVYCSQE ALLQIAENAG GDFRSAINDL QAAASGKEKL EVEDIGTAGR DVKENIFKAM
     QKIFKSTDCK KALESAYGLD ESPEDLVHWI DENLPIQYAR KDGNLEDIKT GFGYLSKADL
     YLGRVKKRQN YRMWRYASML MVCGAALSKT KPYPGFIKYQ QPSLWRRLGQ TRSKRDMRDN
     IASKIGEHSF ESMHYSRNNL LGLYSRMLKD EESAVEVTAN LGLELEELMY LTGSAKASKK
     LQKIYDKAQK LLEEGKNETA DPDFFKAPVP AVDNKQRTLS CPVIIQEEEE KPQKEGSAGK
     SDPPGPHSSE RKQKTLNMGF DSLLETSEKK ENSEKKENSG YLLVDDPKPA EKNLFSFSPS
     LLEKKNFSES VEQKTSSKPS KKGNPANNEP SKQKILDKVT PTESVQDNAG DGAKKAEPKN
     QKTLFDF