ID   RFCL_METBF              Reviewed;         642 AA.
AC   Q46AT6;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Replication factor C large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE            Short=RFC large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE   AltName: Full=Clamp loader large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
GN   Name=rfcL {ECO:0000255|HAMAP-Rule:MF_01508}; OrderedLocusNames=Mbar_A2075;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC       subunits (RfcL). {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01508}.
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DR   EMBL; CP000099; AAZ71006.1; -; Genomic_DNA.
DR   RefSeq; WP_011307052.1; NC_007355.1.
DR   AlphaFoldDB; Q46AT6; -.
DR   SMR; Q46AT6; -.
DR   STRING; 269797.Mbar_A2075; -.
DR   EnsemblBacteria; AAZ71006; AAZ71006; Mbar_A2075.
DR   GeneID; 3626469; -.
DR   KEGG; mba:Mbar_A2075; -.
DR   eggNOG; arCOG00470; Archaea.
DR   HOGENOM; CLU_027255_0_0_2; -.
DR   OMA; GGWTRYG; -.
DR   OrthoDB; 24257at2157; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01508; RfcL; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023935; Rep_factor-C_lsu.
DR   Pfam; PF00004; AAA; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; Nucleotide-binding.
FT   CHAIN           1..642
FT                   /note="Replication factor C large subunit"
FT                   /id="PRO_0000245636"
FT   REGION          432..452
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          464..518
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          568..642
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        499..514
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        587..601
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        605..642
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         50..57
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01508"
SQ   SEQUENCE   642 AA;  71040 MW;  171E0DD22C301462 CRC64;
     MTLAIEWAEK YRPQTLKEIV GNKKAVQYLR TWAEKWLSGI PDRRAVVLHG PAGVGKTSTA
     HALARDLDWE VIELNASDQR TAGVIERVAG SAASMNTFFG GKRLIILDEA DNIHGTADRG
     GMRAIAGIIK NTLQPIVLIA NDIYGLTPTI RNLCLEIKFG SVQSRSMVPA LKKVCESEDI
     LCSPDAIQQI AEGAGGDLRS AINDLQAAAT GRKTLEVEDL STSGRDVKEN IFKAMQRIFK
     STDCKKALEA ARGLDESPED LVHWIDENLP FQYASKDGNL EDIKTGFGYL SKADLYLGRV
     KKRQNYRMWR YASMLMVCGT SVSKTRPYPG FIKYQPPSLW KKMGQIRSKR DLRDNIASKV
     GEHNFESMRY SRNNLLELYS RMLKNEESAI EITAGLGLEL EELIYLSGST KASKKLQKIY
     ERAQELLLEG NEESDGAEFF RTPAPPGNSK EDLSVFSVNN SVEKDGKHSE RLEVPNSRTS
     QGGQKTLNFG FDIPPEISPE TKNSENNTSD TIKPGNLAPD ILAPVTLTPD SFTSDTLIPE
     QDQAEIDDIG IKSDSLTSIS LEHDSLKVKN SSSLPGPVEK EAFSDSEPIE NSISPELSRS
     VKSVNREPAI KKVSDNSEKS EVKVSETPKK VESKTQKTLF DF