ID   RFCL_METHJ              Reviewed;         483 AA.
AC   Q2FQR4;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Replication factor C large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE            Short=RFC large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE   AltName: Full=Clamp loader large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
GN   Name=rfcL {ECO:0000255|HAMAP-Rule:MF_01508}; OrderedLocusNames=Mhun_0916;
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC       subunits (RfcL). {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01508}.
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DR   EMBL; CP000254; ABD40666.1; -; Genomic_DNA.
DR   RefSeq; WP_011447945.1; NC_007796.1.
DR   AlphaFoldDB; Q2FQR4; -.
DR   SMR; Q2FQR4; -.
DR   STRING; 323259.Mhun_0916; -.
DR   EnsemblBacteria; ABD40666; ABD40666; Mhun_0916.
DR   GeneID; 3924142; -.
DR   KEGG; mhu:Mhun_0916; -.
DR   eggNOG; arCOG00470; Archaea.
DR   HOGENOM; CLU_027255_1_0_2; -.
DR   OMA; GGWTRYG; -.
DR   OrthoDB; 24257at2157; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01508; RfcL; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023935; Rep_factor-C_lsu.
DR   Pfam; PF00004; AAA; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..483
FT                   /note="Replication factor C large subunit"
FT                   /id="PRO_0000245638"
FT   REGION          417..483
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        417..445
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         43..50
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01508"
SQ   SEQUENCE   483 AA;  53656 MW;  BBF34F8B3CD19FDF CRC64;
     MDWAEKYRPR HLNEMVGNRE ALHQMSEWAT RWTVESPPLI LYGKPGIGKT SSAWALAHDM
     NWEVVELNAS DQRTKAVIEK VAGGSASTGS LTGAARKLII LDEADNLQGN ADRGGARAIA
     EVIRQARQPL ILIANDLYGL DGTIRNLCTK VQFKALPAKS LVPRLREICS REQLTCSAQA
     LTDIAEQSGG DIRSAVTMLY ASAIGKDTVG EDDVSISAKD SRASIFDLVA ATLGYRQVPS
     LLDMSMSVDE TPDTILQWIE GNLGVLPDRK KTAQAYAALS RADMYLGYTF LTQYYTLWRY
     ATSVMLYGVH DVMKGRPSGY AKIMPPSRWR QMSVAKKQKT IREQLLSDMG TSMHMSASTV
     RNLYLCPISL LAKQFPEQFA KSYDLDVDKL DILIHDPATS KSIIKKIEDE KKQIEKELKK
     KKKEEDAKGK KARGSKKEKE PIVEETPSID SFSSQEPGNP PEQPSTEPVE KDKKSSQSTL
     FSF