ID   RFCL_METST              Reviewed;         534 AA.
AC   Q2NH88;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Replication factor C large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE            Short=RFC large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
DE   AltName: Full=Clamp loader large subunit {ECO:0000255|HAMAP-Rule:MF_01508};
GN   Name=rfcL {ECO:0000255|HAMAP-Rule:MF_01508}; OrderedLocusNames=Msp_0414;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC       subunits (RfcL). {ECO:0000255|HAMAP-Rule:MF_01508}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01508}.
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DR   EMBL; CP000102; ABC56815.1; -; Genomic_DNA.
DR   RefSeq; WP_011406015.1; NC_007681.1.
DR   AlphaFoldDB; Q2NH88; -.
DR   SMR; Q2NH88; -.
DR   STRING; 339860.Msp_0414; -.
DR   EnsemblBacteria; ABC56815; ABC56815; Msp_0414.
DR   GeneID; 41324988; -.
DR   KEGG; mst:Msp_0414; -.
DR   eggNOG; arCOG00470; Archaea.
DR   HOGENOM; CLU_027255_1_1_2; -.
DR   OMA; GGWTRYG; -.
DR   OrthoDB; 24257at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01508; RfcL; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023935; Rep_factor-C_lsu.
DR   Pfam; PF00004; AAA; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..534
FT                   /note="Replication factor C large subunit"
FT                   /id="PRO_0000245637"
FT   REGION          444..534
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        444..467
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        468..482
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        483..507
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45..52
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01508"
SQ   SEQUENCE   534 AA;  60778 MW;  3DE0F8A3D369657F CRC64;
     MKWVEKYAPK KLGDVLGNAK AKAQIEVWAN KWSKGVPQKP LLLMGPPGIG KTTIAHLVGK
     EYFSETIEVN ASDKRSYDII KSSIGEAAQT RSLFHSGYKL LIMDEVDGIS GRDDSGGARA
     VNETIKNSKQ PIILMANDAY SKRLTSIKPK CQGIKFTKVH TNSINAQLKR ICAREDIEYD
     SEALYTLSKE SNGDLRSAIT SLEAIVDNDK KITKDSLSVI AKKDGEQNIF DTVVAVLKSK
     NPEHVAEAMR VNTQPPFLIE LIAENIPREY ERTNEIVKAY EMISLADVNL GRAFRTQNYT
     YWKYAFLFMG RGVAAAKKQT YKKFSRIITP TIYTKLSKSR KNRNLKEQVT QKMSLKLHTS
     PKELEKQLVF YEELFKDNEQ AYDLKQYFKL TDDEVKLFRS RKIPASVEKK RLTKLRKEQE
     LEEKEIQKQK IIDAKKVELK EESKNKKEIK VKTKKDTVED SSKTKSTTKS KKTSKSTPTK
     KVTKTKKSSN STTKNKTESP KNSSKTSSKT SVDDKKTSKK NNKKKSRQTT LFDF