RFCL_METTH
ID RFCL_METTH Reviewed; 479 AA.
AC O26342;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Replication factor C large subunit;
DE Short=RFC large subunit;
DE AltName: Full=Clamp loader large subunit;
DE AltName: Full=MthRFC large subunit;
GN Name=rfcL; OrderedLocusNames=MTH_240;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=10497247; DOI=10.1074/jbc.274.40.28751;
RA Kelman Z., Pietrokovski S., Hurwitz J.;
RT "Isolation and characterization of a split B-type DNA polymerase from the
RT archaeon Methanobacterium thermoautotrophicum deltaH.";
RL J. Biol. Chem. 274:28751-28761(1999).
RN [3]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=10702304; DOI=10.1074/jbc.275.10.7327;
RA Kelman Z., Hurwitz J.;
RT "A unique organization of the protein subunits of the DNA polymerase clamp
RT loader in the archaeon Methanobacterium thermoautotrophicum deltaH.";
RL J. Biol. Chem. 275:7327-7336(2000).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. The complex possesses DNA-dependent ATPase
CC activity which is further stimulated by PCNA. In conjunction with PCNA
CC stimulates DNA synthesis by PolB, relieving inhibition by replication
CC protein A (RPA). {ECO:0000269|PubMed:10497247,
CC ECO:0000269|PubMed:10702304}.
CC -!- SUBUNIT: Heterohexamer composed of four small subunits (RfcS) and two
CC large subunits (RfcL). {ECO:0000269|PubMed:10702304}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC subfamily. {ECO:0000305}.
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DR EMBL; AE000666; AAB84746.1; -; Genomic_DNA.
DR PIR; A69130; A69130.
DR RefSeq; WP_010875879.1; NC_000916.1.
DR AlphaFoldDB; O26342; -.
DR SMR; O26342; -.
DR STRING; 187420.MTH_240; -.
DR EnsemblBacteria; AAB84746; AAB84746; MTH_240.
DR GeneID; 1470201; -.
DR KEGG; mth:MTH_240; -.
DR PATRIC; fig|187420.15.peg.209; -.
DR HOGENOM; CLU_027255_1_0_2; -.
DR OMA; GGWTRYG; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01508; RfcL; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR023935; Rep_factor-C_lsu.
DR Pfam; PF00004; AAA; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT CHAIN 1..479
FT /note="Replication factor C large subunit"
FT /id="PRO_0000135956"
FT REGION 402..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..472
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 54393 MW; E75BFB936C6B5A1D CRC64;
MSWTEKYRPG SFDEVVGNQK VIAEIKEWIK AWKAGKPQKP LLLVGPPGTG KTTLAHIIGK
EFSDTLELNA SDRRSQDALM RSAGEASATR SLFNHDLKLI ILDEVDGIHG NEDRGGVQAI
NRIIKESRHP MVLTANDPYS KRLQSIKPRC RVLNLRKVHT SSIAAALRRI CRAEGIECPD
DVLRELAKRS RGDLRSAIND LEAMAEGEER IGEELLKMGE KDATSNLFDA VRAVLKSRDV
SKVREAMRVD DDPTLVLEFI AENVPREYEK PNEISRAYDM LSRADIFFGR AVRTRNYTYW
RYASELMGPG VALAKDKTYR KFVRYTGSSS FRILGKTRKQ RSLRDSVAAK MAGKMHISPK
VAISMFPYME ILFENDEMAY DISEFLELRD EEIKLFRKRK IKAPKRKKTP RKAEIKVGPL
YSQKKDKGAD KSINDKATDK SAKTPIKSSK KDDRPRDESS SSSDDKKPKE KQTSLFQFS
