RFCL_PYRAB
ID RFCL_PYRAB Reviewed; 479 AA.
AC Q9V2G3; G8ZFU6;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Replication factor C large subunit;
DE Short=RFC large subunit;
DE AltName: Full=Clamp loader large subunit;
DE AltName: Full=PabRFC large subunit;
GN Name=rfcL; OrderedLocusNames=PYRAB01110; ORFNames=PAB0069;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
RN [3]
RP PROTEIN SEQUENCE OF 2-12, FUNCTION, AND SUBUNIT.
RC STRAIN=GE5 / Orsay;
RX PubMed=12417194; DOI=10.1016/s0022-2836(02)01028-8;
RA Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J.,
RA Huebscher U., Raffin J.-P.;
RT "Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi
RT does not need ATP hydrolysis for clamp-loading and contains a functionally
RT conserved RFC PCNA-binding domain.";
RL J. Mol. Biol. 323:795-810(2002).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. The complex possesses DNA-independent ATPase
CC activity. {ECO:0000269|PubMed:12417194}.
CC -!- SUBUNIT: Heterohexamer composed of four small subunits (RfcS) and two
CC large subunits (RfcL). {ECO:0000305|PubMed:12417194}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC subfamily. {ECO:0000305}.
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DR EMBL; AJ248283; CAB49035.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE69487.1; -; Genomic_DNA.
DR PIR; D75198; D75198.
DR RefSeq; WP_010867235.1; NC_000868.1.
DR AlphaFoldDB; Q9V2G3; -.
DR SMR; Q9V2G3; -.
DR IntAct; Q9V2G3; 1.
DR MINT; Q9V2G3; -.
DR STRING; 272844.PAB0069; -.
DR PRIDE; Q9V2G3; -.
DR EnsemblBacteria; CAB49035; CAB49035; PAB0069.
DR GeneID; 1494998; -.
DR KEGG; pab:PAB0069; -.
DR PATRIC; fig|272844.11.peg.124; -.
DR eggNOG; arCOG00470; Archaea.
DR HOGENOM; CLU_027255_1_1_2; -.
DR OMA; GGWTRYG; -.
DR OrthoDB; 24257at2157; -.
DR PhylomeDB; Q9V2G3; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01508; RfcL; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR023935; Rep_factor-C_lsu.
DR Pfam; PF00004; AAA; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Direct protein sequencing; DNA replication;
KW Nucleotide-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:12417194"
FT CHAIN 2..479
FT /note="Replication factor C large subunit"
FT /id="PRO_0000135960"
FT REGION 420..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 50..57
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 55300 MW; A29BB46E376FBF62 CRC64;
MPEVPWVEKY RPRRLSEIIN QEDAISKVKA WIEAWLHGNP PKKKALLLAG PPGSGKTTTV
YALAREYNFE VIELNASDER TYDKIARYVQ AAYTMDILGK RRKIIFLDEA DNIEPSGAPE
IAKLIDRARN PIIMAANHYW EVPKEIRDRA ELVEYKRLTQ RDVINALVRI LKREGITVPK
EVLVEIAKRA SGDLRAAIND LQTVVAGGYE DARYVLAYRD VEKTVFQALG LVFASDNAKR
AKLAMMNLDM SPDEFLLWID ENIPHMYLKP EEMARAYEAI SKADIYLGRA QRTGNYSLWR
YAIDMMTAGV AVAGTKKRGF AKFYPPNTLK MLAESKEERS TRNSIIKKIM SEMHMSKLEA
IETMKIIKTI FEKNLDLAAH FTVFLGLSEK EVEFLAGREN AGTIWGKALA IRRKLKKEEE
KIRKERKEEE KVEVREEKPE EKVEEKREER ETKKEKEKKE EKKAEKKGKQ VTLFDFIKK
