RFCL_SACS2
ID RFCL_SACS2 Reviewed; 405 AA.
AC Q9UXF6;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Replication factor C large subunit;
DE Short=RFC large subunit;
DE AltName: Full=Clamp loader large subunit;
DE AltName: Full=SsoRFC large subunit;
GN Name=rfcL; OrderedLocusNames=SSO0769; ORFNames=C40_005;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=10701121; DOI=10.1139/g99-108;
RA Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C.,
RA Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T.,
RA Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E.,
RA Medina N., Peng X., Penny S.L., She Q., St Jean A., van der Oost J.,
RA Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.;
RT "Gene content and organization of a 281-kbp contig from the genome of the
RT extremely thermophilic archaeon, Sulfolobus solfataricus P2.";
RL Genome 43:116-136(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=10926493; DOI=10.1006/jmbi.2000.3964;
RA Pisani F.M., De Felice M., Carpentieri F., Rossi M.;
RT "Biochemical characterization of a clamp-loader complex homologous to
RT eukaryotic replication factor C from the hyperthermophilic archaeon
RT Sulfolobus solfataricus.";
RL J. Mol. Biol. 301:61-73(2000).
RN [4]
RP FUNCTION, INTERACTION WITH PCNA3, AND SUBUNIT.
RX PubMed=12535540; DOI=10.1016/s1097-2765(02)00824-9;
RA Dionne I., Nookala R.K., Jackson S.P., Doherty A.J., Bell S.D.;
RT "A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus
RT solfataricus.";
RL Mol. Cell 11:275-282(2003).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. The complex possesses DNA-dependent ATPase
CC activity. {ECO:0000269|PubMed:10926493, ECO:0000269|PubMed:12535540}.
CC -!- SUBUNIT: Heteropentamer composed of four small subunits (RfcS) and one
CC large subunit (RfcL). Probably interacts with PCNA subunit PCNA3.
CC {ECO:0000269|PubMed:10926493, ECO:0000269|PubMed:12535540}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcL
CC subfamily. {ECO:0000305}.
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DR EMBL; Y18930; CAB57534.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK41066.1; -; Genomic_DNA.
DR PIR; C90226; C90226.
DR RefSeq; WP_009991358.1; NC_002754.1.
DR AlphaFoldDB; Q9UXF6; -.
DR SMR; Q9UXF6; -.
DR STRING; 273057.SSO0769; -.
DR EnsemblBacteria; AAK41066; AAK41066; SSO0769.
DR GeneID; 44129774; -.
DR KEGG; sso:SSO0769; -.
DR PATRIC; fig|273057.12.peg.768; -.
DR eggNOG; arCOG00470; Archaea.
DR HOGENOM; CLU_027255_1_1_2; -.
DR InParanoid; Q9UXF6; -.
DR OMA; GGWTRYG; -.
DR PhylomeDB; Q9UXF6; -.
DR BRENDA; 3.6.4.B8; 6163.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01508; RfcL; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR023935; Rep_factor-C_lsu.
DR Pfam; PF00004; AAA; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT CHAIN 1..405
FT /note="Replication factor C large subunit"
FT /id="PRO_0000135966"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 405 AA; 46787 MW; D9CF8AF297999E46 CRC64;
MIQWFLKYRP RSLKDVENQD DAKKQLQEWI ESWLNGNSNV KAVLLHGPPG VGKTVLAEAL
AHDYNFELLE MNASDSRKLQ DIKSIAEKAA VYGSIFGTKG KLILLDEVDG INVREDTGAI
QGILELIEKT KYPIIMTAND PWNPGLRELR NKAKMIELSK LGKYPLRRIL KKICQAEKII
CDDEALNYII DSSEGDARYA INILQGIGEG YGKVTLNLVE SLAKRKEREL DPFETLRDIF
WARYAWQAKN AATSAQIDYD MLIRWISENI PIQYDNIEDI WRAFDALSRA SIFLKRAKSG
DWDLLSYAYD MMSSGVAFAE PEKKKPNWKP KWKKYQFPSY IQLLSKSKDI RDTRDEIIKK
LAIHSSFNKA LNDTYPFFLL FYKKYDKHLN LNTKEKEYLS SISKS
