ID   RFCS_METKA              Reviewed;         635 AA.
AC   Q8TZC4;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Replication factor C small subunit;
DE            Short=RFC small subunit;
DE   AltName: Full=Clamp loader small subunit;
DE   Contains:
DE     RecName: Full=Mkn RFC intein;
GN   Name=rfcS; OrderedLocusNames=MK0006;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. {ECO:0000250}.
CC   -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC       subunits (RfcL). {ECO:0000250}.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcS
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE009439; AAM01223.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TZC4; -.
DR   SMR; Q8TZC4; -.
DR   STRING; 190192.MK0006; -.
DR   MEROPS; N10.007; -.
DR   EnsemblBacteria; AAM01223; AAM01223; MK0006.
DR   KEGG; mka:MK0006; -.
DR   PATRIC; fig|190192.8.peg.6; -.
DR   HOGENOM; CLU_015698_2_0_2; -.
DR   OMA; NWRYEFR; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009378; F:four-way junction helicase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR013748; Rep_factorC_C.
DR   InterPro; IPR008824; RuvB-like_N.
DR   Pfam; PF08542; Rep_fac_C; 1.
DR   Pfam; PF05496; RuvB_N; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF48019; SSF48019; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; DNA replication; Nucleotide-binding;
KW   Protein splicing; Reference proteome.
FT   CHAIN           1..82
FT                   /note="Replication factor C small subunit, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030368"
FT   CHAIN           83..387
FT                   /note="Mkn RFC intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030369"
FT   CHAIN           388..635
FT                   /note="Replication factor C small subunit, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030370"
FT   BINDING         51..58
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   635 AA;  71586 MW;  9AC69359719E08C5 CRC64;
     MAEHELRVLE IPWVEKYRPK RLDDIVDQEH VVERLKAYVN RGDMPNLLFA GPPGTGKTTA
     ALCLARELFG EHWRDNFLEL NASVSADTPI LVRRGGEVLR VTFEDLDSWY FGDRGGEYVD
     VSDLEVLTVD RNFRVTWARV SKLIRHRARK ILRVHLEDGT IELTGNHAVM VLDEGGLRAV
     KASEIEEGSF LLSFVAELDE QPTDGGTVVT SVGSGSRVSD TTYELPVEVR VELLRELADD
     GVIEASEDVS VDLAWLARIS GVESRVTDDG VELVWETRTG DLLPADPVLK LVERLESDLV
     DDLESWVFDG RVSKEAVRKV LSSVDAKNLR GDARRAYRML RTLVRSDVHA VKVEDLDVMD
     YDGYVYDVSV PGNEMFFAGE VPVLLHNSDE RGIDVIRTKV KNFARTRPMG GARFKIIFLD
     EADNLTRDSQ QALRRIMEMY SDACRFILAA NYSSAIIDPI QSRCVVFKFT KLPESAIKER
     LRKIAESEGV EITEDALDAI VYVSEGDMRR AINVLQAAAA LGREIDEDTV FQIAATARPE
     EVREMIHHAW NGDFERAREL LHELLTRYGM SGEDVVRQVH REIFDMDEIP EEAIPELVNA
     VGDFEYRLIR GSDERIQLEA LLARIHALGN EYSGG