ID   RFCS_METTH              Reviewed;         321 AA.
AC   O26343;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Replication factor C small subunit;
DE            Short=RFC small subunit;
DE   AltName: Full=Clamp loader small subunit;
DE   AltName: Full=MthRFC small subunit;
GN   Name=rfcS; OrderedLocusNames=MTH_241;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   FUNCTION.
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=10497247; DOI=10.1074/jbc.274.40.28751;
RA   Kelman Z., Pietrokovski S., Hurwitz J.;
RT   "Isolation and characterization of a split B-type DNA polymerase from the
RT   archaeon Methanobacterium thermoautotrophicum deltaH.";
RL   J. Biol. Chem. 274:28751-28761(1999).
RN   [3]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=10702304; DOI=10.1074/jbc.275.10.7327;
RA   Kelman Z., Hurwitz J.;
RT   "A unique organization of the protein subunits of the DNA polymerase clamp
RT   loader in the archaeon Methanobacterium thermoautotrophicum deltaH.";
RL   J. Biol. Chem. 275:7327-7336(2000).
CC   -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC       sliding clamp onto DNA. The complex possesses DNA-dependent ATPase
CC       activity which is further stimulated by PCNA. In conjunction with PCNA
CC       stimulates DNA synthesis by PolB, relieving inhibition by replication
CC       protein A (RPA). {ECO:0000269|PubMed:10497247,
CC       ECO:0000269|PubMed:10702304}.
CC   -!- SUBUNIT: Heterohexamer composed of four small subunits (RfcS) and two
CC       large subunits (RfcL). {ECO:0000269|PubMed:10702304}.
CC   -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcS
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE000666; AAB84747.1; -; Genomic_DNA.
DR   PIR; B69130; B69130.
DR   AlphaFoldDB; O26343; -.
DR   SMR; O26343; -.
DR   STRING; 187420.MTH_241; -.
DR   EnsemblBacteria; AAB84747; AAB84747; MTH_241.
DR   KEGG; mth:MTH_241; -.
DR   PATRIC; fig|187420.15.peg.210; -.
DR   HOGENOM; CLU_042324_2_1_2; -.
DR   OMA; SCNYSSQ; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005663; C:DNA replication factor C complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01509; RfcS; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023748; Rep_factor-C_ssu_arc.
DR   InterPro; IPR013748; Rep_factorC_C.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF08542; Rep_fac_C; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF48019; SSF48019; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..321
FT                   /note="Replication factor C small subunit"
FT                   /id="PRO_0000135977"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   321 AA;  36788 MW;  1E8059637839EDC5 CRC64;
     MIIMNGPWVE KYRPQKLDDI VGQEHIIPRL KRYVEEKSMP NLMFTGPAGV GKTTAALALA
     REILGEYWRQ NFLELNASDA RGIDTVRTSI KNFCRLKPVG APFRIIFLDE VDNMTKDAQH
     ALRREMEMYT KTSSFILSCN YSSKIIDPIQ SRCAIFRFLP LKGHQIIKRL EYIAEKENLE
     YEAHALETIV YFAEGDLRKA INLLQSAASL GEKITESSIY DVVSRARPKD VRKMIKTILD
     GKFMEARDML REIMVLQGIS GEDMVTQIYQ ELSRLAMEGE VDGDRYVGLI DAIGEYDFRI
     REGANPRIQL EALLARFLEH A