RFCS_SACS2
ID RFCS_SACS2 Reviewed; 330 AA.
AC Q9UXF5;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Replication factor C small subunit;
DE Short=RFC small subunit;
DE AltName: Full=Clamp loader small subunit;
DE AltName: Full=SsoRFC small subunit;
GN Name=rfcS; OrderedLocusNames=SSO0768; ORFNames=C40_004;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=10701121; DOI=10.1139/g99-108;
RA Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C.,
RA Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T.,
RA Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E.,
RA Medina N., Peng X., Penny S.L., She Q., St Jean A., van der Oost J.,
RA Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.;
RT "Gene content and organization of a 281-kbp contig from the genome of the
RT extremely thermophilic archaeon, Sulfolobus solfataricus P2.";
RL Genome 43:116-136(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=10926493; DOI=10.1006/jmbi.2000.3964;
RA Pisani F.M., De Felice M., Carpentieri F., Rossi M.;
RT "Biochemical characterization of a clamp-loader complex homologous to
RT eukaryotic replication factor C from the hyperthermophilic archaeon
RT Sulfolobus solfataricus.";
RL J. Mol. Biol. 301:61-73(2000).
RN [4]
RP FUNCTION, INTERACTION WITH PCNA1 AND PCNA2, AND SUBUNIT.
RX PubMed=12535540; DOI=10.1016/s1097-2765(02)00824-9;
RA Dionne I., Nookala R.K., Jackson S.P., Doherty A.J., Bell S.D.;
RT "A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus
RT solfataricus.";
RL Mol. Cell 11:275-282(2003).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. The complex possesses DNA-dependent ATPase
CC activity. {ECO:0000269|PubMed:10926493, ECO:0000269|PubMed:12535540}.
CC -!- SUBUNIT: Heteropentamer composed of four small subunits (RfcS) and one
CC large subunit (RfcL). A homotetramer of this subunit interacts with
CC PCNA heterodimer PCNA1-PCNA2. {ECO:0000269|PubMed:10926493,
CC ECO:0000269|PubMed:12535540}.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcS
CC subfamily. {ECO:0000305}.
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DR EMBL; Y18930; CAB57535.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK41065.1; -; Genomic_DNA.
DR PIR; B90226; B90226.
DR RefSeq; WP_009991357.1; NC_002754.1.
DR AlphaFoldDB; Q9UXF5; -.
DR SMR; Q9UXF5; -.
DR STRING; 273057.SSO0768; -.
DR PRIDE; Q9UXF5; -.
DR EnsemblBacteria; AAK41065; AAK41065; SSO0768.
DR GeneID; 44129773; -.
DR KEGG; sso:SSO0768; -.
DR PATRIC; fig|273057.12.peg.767; -.
DR eggNOG; arCOG00469; Archaea.
DR HOGENOM; CLU_042324_2_1_2; -.
DR InParanoid; Q9UXF5; -.
DR OMA; SCNYSSQ; -.
DR PhylomeDB; Q9UXF5; -.
DR BRENDA; 3.6.4.B8; 6163.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005663; C:DNA replication factor C complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003689; F:DNA clamp loader activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01509; RfcS; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR023748; Rep_factor-C_ssu_arc.
DR InterPro; IPR013748; Rep_factorC_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF08542; Rep_fac_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; Nucleotide-binding; Reference proteome.
FT CHAIN 1..330
FT /note="Replication factor C small subunit"
FT /id="PRO_0000135984"
FT BINDING 48..55
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 330 AA; 37741 MW; 89258D375BEEA23E CRC64;
MSTKVEEILW AEKYRPKTLD DIVNQREIID RLKKFVKEKN MPHLLFAGPP GTGKTTAALA
LVHDLYGDNY TEYFLELNAS DERGIDVIRN KVKEFARTVI PGDIPFKVVL LDEADNMTAD
AQQALRRTME LYTENTRFIL ACNYLSKIIE PIQSRTALFR FYPLKKEDVV NRLIYIAKNE
KAEYDQKALE TIYDITMGDM RKSINILQAA SAYGKISVEA VFKVLGLAQP KEVREMINLA
LQGKFTQARD KLRTLLITYG LSGEDIVKQI HREITSSEIQ ISEELRVLLL DYIGETEFRI
IEGADDEIQL SALLAKMAIY GNKYIGSENR
