RFCS_THEKO
ID RFCS_THEKO Reviewed; 866 AA.
AC Q5JHP2;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Replication factor C small subunit;
DE Short=RFC small subunit;
DE AltName: Full=Clamp loader small subunit;
DE Contains:
DE RecName: Full=Pko RFC intein;
GN Name=rfcS; OrderedLocusNames=TK2218;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
RN [2]
RP PROTEIN SEQUENCE OF 1-6, AND FUNCTION.
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=14646196; DOI=10.1271/bbb.67.2373;
RA Kitabayashi M., Nishiya Y., Esaka M., Itakura M., Imanaka T.;
RT "Gene cloning and function analysis of replication factor C from
RT Thermococcus kodakaraensis KOD1.";
RL Biosci. Biotechnol. Biochem. 67:2373-2380(2003).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. {ECO:0000305|PubMed:14646196}.
CC -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC subunits (RfcL). {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the potential ATP-binding site.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcS
CC subfamily. {ECO:0000305}.
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DR EMBL; AP006878; BAD86407.1; -; Genomic_DNA.
DR RefSeq; WP_011251168.1; NC_006624.1.
DR AlphaFoldDB; Q5JHP2; -.
DR SMR; Q5JHP2; -.
DR STRING; 69014.TK2218; -.
DR EnsemblBacteria; BAD86407; BAD86407; TK2218.
DR GeneID; 3234370; -.
DR KEGG; tko:TK2218; -.
DR PATRIC; fig|69014.16.peg.2174; -.
DR eggNOG; arCOG00469; Archaea.
DR eggNOG; arCOG03154; Archaea.
DR HOGENOM; CLU_015698_0_0_2; -.
DR InParanoid; Q5JHP2; -.
DR OMA; QFADNTR; -.
DR OrthoDB; 37207at2157; -.
DR PhylomeDB; Q5JHP2; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005663; C:DNA replication factor C complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0009378; F:four-way junction helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013748; Rep_factorC_C.
DR InterPro; IPR008824; RuvB-like_N.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF01381; HTH_3; 1.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR Pfam; PF08542; Rep_fac_C; 1.
DR Pfam; PF05496; RuvB_N; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Autocatalytic cleavage; Direct protein sequencing;
KW DNA replication; Nucleotide-binding; Protein splicing; Reference proteome.
FT CHAIN 1..59
FT /note="Replication factor C small subunit, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030382"
FT CHAIN 60..599
FT /note="Pko RFC intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030383"
FT CHAIN 600..866
FT /note="Replication factor C small subunit, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030384"
FT DOMAIN 183..313
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ SEQUENCE 866 AA; 99691 MW; 0E9386BB07638F0D CRC64;
MSEEVKEVKI LEKPWVEKYR PQRLEDIVGQ DHIVKRLKHY VKTGSMPHLL FAGPPGVGKC
LTGDAKVIAN GRLFELGELV EKVSKGRFGP TPVEGLKVLG IDEDGKLREF EVQYVYKDRA
ERLIKVRTRL GRELKVTPYH PLLVNRKNGE IMWVKAEELR PGDRLAVPRF LPAIAEEDPL
AEWLGYFIGD GHADSKNKVI TFTNTDPSLR QRFMELTERL FPDAKIRERI HKNRAPDVYV
NSRRAWELVS SLGLAGRKAD KVYIPEKGWE GIRSFLRAYF DCDAGVDKNA VVLATASREM
AEQVTYALAG FGITSKIREK KVRGKTYYHV TISGSENLER FLSEIGFSHR EKLERTLKLV
KKPNPNLDSL NVNYELISYV RDRLKLNFSD DKRSWSHRKA RKISWELMKE IYYRLDELER
LKESLSRSIL IDWNEMAERR KEIAEKTGIR ADRLLEYIKG KRKPSLRNYI KIAKALGIDL
EPTINAMRVF ARKYSSYAEI GRKLGTWNSS VRIILESNTE KIKELEEIRK IELELIGEIL
SDEKLKEGVA YLIFLSQNEL YWDEITEVKE LKGDFVIYDL HVPGYHNFIA GNMPTVVHNT
TAALALAREL FGENWRHNFL ELNASDERGI NVIREKVKEF ARTKPIGGAS FKIIFLDEAD
ALTQDAQQAL RRTMEMFSNN VRFILSCNYS SKIIEPIQSR CAIFRFRPLR DEDIAKRIRY
IAENEGLELT EEGLQAILYV AEGDLRRAIN VLQAAAALDT KITDENVFLV ASRARPEDVR
EMMTLALEGN FLKAREKLRD ILLRQGLSGE DVLIQMHKEV FNLPIPEDKK VALADKIGEY
NFRLVEGANE MIQLEALLAQ FTIMGK