RFC_ECOLI
ID RFC_ECOLI Reviewed; 388 AA.
AC P37748;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=O-antigen polymerase;
GN Name=rfc; Synonyms=yefF; OrderedLocusNames=b2035, JW2020;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7517390; DOI=10.1128/jb.176.13.4133-4143.1994;
RA Yao Z., Valvano M.A.;
RT "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region
RT (rfb) of Escherichia coli K-12 W3110: identification of genes that confer
RT group 6 specificity to Shigella flexneri serotypes Y and 4a.";
RL J. Bacteriol. 176:4133-4143(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / WG1;
RX PubMed=7517391; DOI=10.1128/jb.176.13.4144-4156.1994;
RA Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M.,
RA Redmond J.W., Lindquist L., Reeves P.R.;
RT "Structure of the O antigen of Escherichia coli K-12 and the sequence of
RT its rfb gene cluster.";
RL J. Bacteriol. 176:4144-4156(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: May link the O-antigen tetrasaccharide units into long
CC chains, giving rise to typical smooth LPS.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
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DR EMBL; U03041; AAC31633.1; -; Genomic_DNA.
DR EMBL; U09876; AAB88404.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75096.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15877.1; -; Genomic_DNA.
DR PIR; I69644; I69644.
DR RefSeq; NP_416539.1; NC_000913.3.
DR RefSeq; WP_000639866.1; NZ_LN832404.1.
DR AlphaFoldDB; P37748; -.
DR BioGRID; 4261358; 168.
DR STRING; 511145.b2035; -.
DR TCDB; 9.B.67.2.1; the o-antigen polymerase (oap) family.
DR jPOST; P37748; -.
DR PaxDb; P37748; -.
DR PRIDE; P37748; -.
DR EnsemblBacteria; AAC75096; AAC75096; b2035.
DR EnsemblBacteria; BAA15877; BAA15877; BAA15877.
DR GeneID; 945179; -.
DR KEGG; ecj:JW2020; -.
DR KEGG; eco:b2035; -.
DR PATRIC; fig|1411691.4.peg.216; -.
DR EchoBASE; EB1925; -.
DR eggNOG; ENOG502ZGEK; Bacteria.
DR HOGENOM; CLU_711211_0_0_6; -.
DR OMA; TWNLEVY; -.
DR BioCyc; EcoCyc:EG11982-MON; -.
DR UniPathway; UPA00030; -.
DR PRO; PR:P37748; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
PE 4: Predicted;
KW Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..388
FT /note="O-antigen polymerase"
FT /id="PRO_0000097298"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 388 AA; 44744 MW; F6B6A10DBA0199FB CRC64;
MIYLVISVFL ITAFICLYLK KDIFYPAVCV NIIFALVLLG YEITSDIYAF QLNDATLIFL
LCNVLTFTLS CLLTESVLDL NIRKVNNAIY SIPSKKVHNV GLLVISFSMI YICMRLSNYQ
FGTSLLSYMN LIRDADVEDT SRNFSAYMQP IILTTFALFI WSKKFTNTKV SKTFTLLVFI
VFIFAIILNT GKQIVFMVII SYAFIVGVNR VKHYVYLITA VGVLFSLYML FLRGLPGGMA
YYLSMYLVSP IIAFQEFYFQ QVSNSASSHV FWFFERLMGL LTGGVSMSLH KEFVWVGLPT
NVYTAFSDYV YISAELSYLM MVIHGCISGV LWRLSRNYIS VKIFYSYFIY TFSFIFYHES
FMTNISSWIQ ITLCIIVFSQ FLKAQKIK