ID   RFC_SHIDY               Reviewed;         380 AA.
AC   Q03584;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=O-antigen polymerase;
GN   Name=rfc;
OS   Shigella dysenteriae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2469933; DOI=10.1016/0882-4010(86)90056-2;
RA   Sturm S., Jann B., Jann K., Fortnagel P., Timmis K.N.;
RT   "Genetic and biochemical analysis of Shigella dysenteriae 1 O antigen
RT   polysaccharide biosynthesis in Escherichia coli K-12: structure and
RT   functions of the rfb gene cluster.";
RL   Microb. Pathog. 1:307-324(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7692219; DOI=10.1111/j.1365-2958.1993.tb01700.x;
RA   Klena J.D., Schnaitman C.A.;
RT   "Function of the rfb gene cluster and the rfe gene in the synthesis of O
RT   antigen by Shigella dysenteriae 1.";
RL   Mol. Microbiol. 9:393-402(1993).
CC   -!- FUNCTION: May link the O-antigen tetrasaccharide units into long
CC       chains, giving rise to typical smooth LPS.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
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DR   EMBL; L07293; AAA16935.1; -; Unassigned_DNA.
DR   PIR; S34964; S34964.
DR   AlphaFoldDB; Q03584; -.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..380
FT                   /note="O-antigen polymerase"
FT                   /id="PRO_0000097302"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        229..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   380 AA;  43732 MW;  557DDC895215E18E CRC64;
     MTYFTGFILI LFAIIIKRLT PSQSKKNIVL IANAFWGILL VGYTFNEQYF VPLSATTLFF
     ILAFLFFFSM TYILIARSGR VVFSFGTGFI ESKYIYWFAG MINIISICFG IILLYNNHFS
     LKVMREGILD GSISGFGLGI SLPLSFCCMY LARHENKKNY FYCFTLLSFL LAVLSTSKIF
     LILFLVYIVG INSYVSKKKL LIYGVFVFGL FALSSIILGK FSSDPEGKII SAIFDTLRVY
     LFSGLAAFNL YVEKNATLPE NLLLYPFKEV WGTTKDIPKT DILPWINIGV WDTNVYTAFA
     PWYQSLGLYA AIIIGILLGF YYGIWFSFRQ NLAVGFYQTF LCFPLLMLFF QEHYLLSWKM
     HFIYFLCAIL LAMRKALEYE