ID   RFC_SHIFL               Reviewed;         382 AA.
AC   P37784;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=O-antigen polymerase;
GN   Name=rfc; OrderedLocusNames=SF2097, S2219;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PE577 / Serotype 2a;
RX   PubMed=7507920; DOI=10.1128/jb.176.3.733-747.1994;
RA   Morona R., Mavris M., Fallarino A., Manning P.A.;
RT   "Characterization of the rfc region of Shigella flexneri.";
RL   J. Bacteriol. 176:733-747(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: May link the O-antigen tetrasaccharide units into long
CC       chains, giving rise to typical smooth LPS.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
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DR   EMBL; X71970; CAA50774.1; -; Genomic_DNA.
DR   EMBL; AE005674; AAN43636.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17465.1; -; Genomic_DNA.
DR   PIR; C36966; C36966.
DR   RefSeq; NP_707929.1; NC_004337.2.
DR   RefSeq; WP_001059773.1; NZ_WPGV01000030.1.
DR   AlphaFoldDB; P37784; -.
DR   STRING; 198214.SF2097; -.
DR   EnsemblBacteria; AAN43636; AAN43636; SF2097.
DR   EnsemblBacteria; AAP17465; AAP17465; S2219.
DR   GeneID; 1025297; -.
DR   KEGG; sfl:SF2097; -.
DR   KEGG; sft:NCTC1_02305; -.
DR   KEGG; sfx:S2219; -.
DR   PATRIC; fig|198214.7.peg.2505; -.
DR   HOGENOM; CLU_723087_0_0_6; -.
DR   OMA; FTANICS; -.
DR   OrthoDB; 2060266at2; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..382
FT                   /note="O-antigen polymerase"
FT                   /id="PRO_0000097303"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        348..368
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   382 AA;  43724 MW;  DCE02BB41619156A CRC64;
     MNNINKIFIT FLCIELIIGG GGRLLEPLGI FPLRYLLFVF SFILLIFNLV TFNFSITQKC
     VSLFIWLLLF PFYGFFVGLL AGNKINDILF DVQPYLFMLS LIYLFTLRYT LKVFSCEIFI
     KIVNAFALYG SLLYISYIIL LNFGLLNFNL IYEHLSLTSE FFFRPDGAFF SKSFYFFGVG
     AIISFVDKKY LKCLIIVLAI LLTESRGVLL FTTLSLLLAS FKLHKLYLNT IIIILGSVLF
     IIMLYMVGSR SEDSDSVRFN DLYFYYKNVD LATFLFGRGF GSFILDRLRI EIVPLEILQK
     TGVIGVFISL VPMLLIFLKG YFLNSTKTSL MMSLILFFSI TVSITNPFLF TPMGIFIIGV
     VVLWVFSIEN IQISNNLTSG AK