RFG1_CANAL
ID RFG1_CANAL Reviewed; 600 AA.
AC Q5A220; A0A1D8PSA0; Q5A271;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 3.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Repressor of filamentous growth 1;
GN Name=RFG1; Synonyms=ROX1; OrderedLocusNames=CAALFM_CR02640WA;
GN ORFNames=CaO19.10341, CaO19.2823;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11290707; DOI=10.1093/genetics/157.4.1503;
RA Khalaf R.A., Zitomer R.S.;
RT "The DNA binding protein Rfg1 is a repressor of filamentation in Candida
RT albicans.";
RL Genetics 157:1503-1512(2001).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11259598; DOI=10.1128/mcb.21.7.2496-2505.2001;
RA Kadosh D., Johnson A.D.;
RT "Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator
RT Rox1, controls filamentous growth and virulence in Candida albicans.";
RL Mol. Cell. Biol. 21:2496-2505(2001).
RN [6]
RP FUNCTION.
RX PubMed=15814840; DOI=10.1091/mbc.e05-01-0073;
RA Kadosh D., Johnson A.D.;
RT "Induction of the Candida albicans filamentous growth program by relief of
RT transcriptional repression: a genome-wide analysis.";
RL Mol. Biol. Cell 16:2903-2912(2005).
RN [7]
RP INDUCTION.
RX PubMed=17880264; DOI=10.1371/journal.pbio.0050256;
RA Zordan R.E., Miller M.G., Galgoczy D.J., Tuch B.B., Johnson A.D.;
RT "Interlocking transcriptional feedback loops control white-opaque switching
RT in Candida albicans.";
RL PLoS Biol. 5:E256-E256(2007).
RN [8]
RP FUNCTION.
RX PubMed=20656914; DOI=10.1128/ec.00088-10;
RA Cleary I.A., Mulabagal P., Reinhard S.M., Yadev N.P., Murdoch C.,
RA Thornhill M.H., Lazzell A.L., Monteagudo C., Thomas D.P., Saville S.P.;
RT "Pseudohyphal regulation by the transcription factor Rfg1p in Candida
RT albicans.";
RL Eukaryot. Cell 9:1363-1373(2010).
CC -!- FUNCTION: Transcription regulator that functions in both the positive
CC and negative regulation of filamentous growth, depending upon the
CC environmental conditions. Recruits the TUP1/SSN6 general repression
CC complex to achieve repression. Regulates genes encoding cell wall
CC components that are specifically expressed in the filamentous forms
CC such as HWP1, RBT1, HYR1, ECE1, ALS1, RBT4 and RBT5.
CC {ECO:0000269|PubMed:11259598, ECO:0000269|PubMed:11290707,
CC ECO:0000269|PubMed:15814840, ECO:0000269|PubMed:20656914}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}.
CC -!- INDUCTION: Expression is regulated by WOR1.
CC {ECO:0000269|PubMed:17880264}.
CC -!- DISRUPTION PHENOTYPE: Leads to constitutive hyphal growth and
CC avirulence in a mouse model. {ECO:0000269|PubMed:11259598,
CC ECO:0000269|PubMed:11290707}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP017630; AOW31008.1; -; Genomic_DNA.
DR RefSeq; XP_715755.2; XM_710662.2.
DR AlphaFoldDB; Q5A220; -.
DR SMR; Q5A220; -.
DR STRING; 237561.Q5A220; -.
DR GeneID; 3642570; -.
DR KEGG; cal:CAALFM_CR02640WA; -.
DR CGD; CAL0000179598; RFG1.
DR VEuPathDB; FungiDB:CR_02640W_A; -.
DR eggNOG; KOG0527; Eukaryota.
DR HOGENOM; CLU_032221_0_0_1; -.
DR OrthoDB; 1641977at2759; -.
DR Proteomes; UP000000559; Chromosome R.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..600
FT /note="Repressor of filamentous growth 1"
FT /id="PRO_0000426083"
FT DNA_BIND 212..281
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 112..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 564..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..473
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..549
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 600 AA; 64967 MW; EA96C229F5B4CAF7 CRC64;
MSTAIYYSTH NNMHTNDDIT NGSGSTTSGN PGANPSNPNH TNAGATINNN SNIGLNSANK
NHTTLLPSLA HIMPSNSNNT TNSGSSIDIS STLINPYSSG AAAAAAAVGG GNGGYYHPPP
PHSQQQQQQQ FDTSYSNQTT PTSAYTNFNN DSTHSPTDIS MQQNMNNLAS SSGNMSIGGG
IDGNFSHNNN NNNNNNNNNN NKCICKSKVN KIPRPRNAFI LFRQKYHQMV LDEGTVIRTN
PEVSRELGRR WRGLSPQEKE HWNNLAEEEK KNHAKKYPGY RYTPRRNGRN KNCPVCKNKP
LPNNKSNSIS GMSGSGGGGG SISGASSLSG GLTSRDNSIT NANAIDYQQQ QLQQQQQQQQ
QQAIQFQSLP PDQYQQLIKQ QQQQLQLQAQ LNGGGGGGGI TSNPQTIPQY ITNGNYPSYI
ISPNPYSTTS TTAPTTTTTT TTNASSIGLS VPPTATTTST SSQPTSANSQ LHFYEAEKLS
PVSSTHHQSS ISEIAAQQQQ QQQQQQFMYN TNYSTIPPNN TTTMQQHSAG TGNDYSLNGN
NSGNTGYDNR YGYGQPMIIT GQSQQGLQVQ QQGQHYNSGL HAAQHYQQQQ QQHHQQQPPQ
