RFM1_YEAST
ID RFM1_YEAST Reviewed; 310 AA.
AC Q12192; D6W2X8;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Repression factor of MSEs protein 1;
GN Name=RFM1; OrderedLocusNames=YOR279C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8896271;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b%3c1059::aid-yea994%3e3.0.co;2-7;
RA Cheret G., Bernardi A., Sor F.J.;
RT "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of
RT Saccharomyces cerevisiae.";
RL Yeast 12:1059-1064(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND INTERACTION WITH SUM1 AND HST1.
RX PubMed=12612074; DOI=10.1128/mcb.23.6.2009-2016.2003;
RA McCord R., Pierce M., Xie J., Wonkatal S., Mickel C., Vershon A.K.;
RT "Rfm1, a novel tethering factor required to recruit the hst1 histone
RT deacetylase for repression of middle sporulation genes.";
RL Mol. Cell. Biol. 23:2009-2016(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Tethering factor required for histone deacetylase HST1-
CC mediated repression. Probably involved in targeting HST1 to a subset of
CC SUM1-regulated genes. {ECO:0000269|PubMed:12612074}.
CC -!- SUBUNIT: Interacts directly with HST1 and SUM1. Required for the
CC interaction between HST1 and SUM1. {ECO:0000269|PubMed:12612074}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 861 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X89633; CAA61784.1; -; Genomic_DNA.
DR EMBL; Z75187; CAA99505.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11044.1; -; Genomic_DNA.
DR PIR; S67181; S67181.
DR RefSeq; NP_014922.3; NM_001183698.3.
DR AlphaFoldDB; Q12192; -.
DR BioGRID; 34667; 100.
DR ComplexPortal; CPX-1384; SUM1-RFM1-HST1 histone deacetylase complex.
DR DIP; DIP-4430N; -.
DR IntAct; Q12192; 9.
DR MINT; Q12192; -.
DR STRING; 4932.YOR279C; -.
DR CarbonylDB; Q12192; -.
DR iPTMnet; Q12192; -.
DR PaxDb; Q12192; -.
DR PRIDE; Q12192; -.
DR EnsemblFungi; YOR279C_mRNA; YOR279C; YOR279C.
DR GeneID; 854453; -.
DR KEGG; sce:YOR279C; -.
DR SGD; S000005805; RFM1.
DR VEuPathDB; FungiDB:YOR279C; -.
DR eggNOG; ENOG502S1B3; Eukaryota.
DR HOGENOM; CLU_055872_1_0_1; -.
DR InParanoid; Q12192; -.
DR OMA; PEKQMRT; -.
DR BioCyc; YEAST:G3O-33766-MON; -.
DR PRO; PR:Q12192; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12192; protein.
DR GO; GO:0000118; C:histone deacetylase complex; IPI:SGD.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IPI:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0032298; P:positive regulation of DNA-templated DNA replication initiation; IGI:SGD.
DR GO; GO:0030174; P:regulation of DNA-templated DNA replication initiation; IC:ComplexPortal.
DR GO; GO:0043937; P:regulation of sporulation; IC:ComplexPortal.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IC:ComplexPortal.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..310
FT /note="Repression factor of MSEs protein 1"
FT /id="PRO_0000097309"
FT REGION 83..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 192..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..219
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 215
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 310 AA; 35195 MW; A3C6D0D33676B6F7 CRC64;
MSTNTEIIKN SDLQSLINDK RRLINEIKDF NKSIKPLEFE SYQDYFLIKT FKKGISASGH
VDIDSLRNKE YGIYYKKIKR NSTQEVGEPI PRNTSSSSSS TRSNSSADIS DTEYSGENTP
TTTGAASRRR RTRSRAIQRE NSLPASLPSI SEANANNDDV TISEINGSEL PFPIPISEVE
NIDIASDITE RDGIRRRSSR ISERDKRRSQ SRLGSEEDEE GDGHDGDEGE TKIQDLYESL
VPKILESRRR SDWILPPKAR YTPEKQMRTK PSFKSIKINE LVGNKRIRSI LSRFEGGVAG
IRKRDWDSTQ
