ID   RFR32_CROS5             Reviewed;         179 AA.
AC   B1WVN5;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Pentapeptide repeat protein Rfr32 {ECO:0000303|PubMed:17075135};
DE   Flags: Precursor;
GN   Name=rfr32 {ECO:0000303|PubMed:17075135};
GN   OrderedLocusNames=cce_1272 {ECO:0000312|EMBL:ACB50622.1};
OS   Crocosphaera subtropica (strain ATCC 51142 / BH68) (Cyanothece sp. (strain
OS   ATCC 51142)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Crocosphaera; Crocosphaera subtropica.
OX   NCBI_TaxID=43989;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51142 / BH68;
RX   PubMed=18812508; DOI=10.1073/pnas.0805418105;
RA   Welsh E.A., Liberton M., Stoeckel J., Loh T., Elvitigala T., Wang C.,
RA   Wollam A., Fulton R.S., Clifton S.W., Jacobs J.M., Aurora R., Ghosh B.K.,
RA   Sherman L.A., Smith R.D., Wilson R.K., Pakrasi H.B.;
RT   "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium
RT   important in the marine nitrogen cycle.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:15094-15099(2008).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS) OF 42-179, SUBUNIT, SUBCELLULAR
RP   LOCATION, DOMAIN, AND DISULFIDE BOND.
RC   STRAIN=ATCC 51142 / BH68;
RX   PubMed=17075135; DOI=10.1110/ps.062407506;
RA   Buchko G.W., Ni S., Robinson H., Welsh E.A., Pakrasi H.B., Kennedy M.A.;
RT   "Characterization of two potentially universal turn motifs that shape the
RT   repeated five-residues fold--crystal structure of a lumenal pentapeptide
RT   repeat protein from Cyanothece 51142.";
RL   Protein Sci. 15:2579-2595(2006).
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:17075135}.
CC   -!- SUBCELLULAR LOCATION: Cellular thylakoid lumen
CC       {ECO:0000305|PubMed:17075135}.
CC   -!- DOMAIN: Forms a right-handed beta-helix with 5 complete coils that
CC       stack upon each other. {ECO:0000269|PubMed:17075135}.
CC   -!- SIMILARITY: Belongs to the pentapeptide repeat protein family.
CC       {ECO:0000305}.
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DR   EMBL; CP000806; ACB50622.1; -; Genomic_DNA.
DR   PDB; 2F3L; X-ray; 2.11 A; A=42-179.
DR   PDB; 2G0Y; X-ray; 2.30 A; A=42-179.
DR   PDBsum; 2F3L; -.
DR   PDBsum; 2G0Y; -.
DR   AlphaFoldDB; B1WVN5; -.
DR   SMR; B1WVN5; -.
DR   STRING; 43989.cce_1272; -.
DR   EnsemblBacteria; ACB50622; ACB50622; cce_1272.
DR   KEGG; cyt:cce_1272; -.
DR   eggNOG; COG1357; Bacteria.
DR   HOGENOM; CLU_066336_3_0_3; -.
DR   OMA; TNAMLMQ; -.
DR   Proteomes; UP000001203; Chromosome circular.
DR   GO; GO:0031979; C:plasma membrane-derived thylakoid lumen; IEA:UniProtKB-SubCell.
DR   InterPro; IPR001646; 5peptide_repeat.
DR   Pfam; PF00805; Pentapeptide; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Reference proteome; Signal; Thylakoid.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..179
FT                   /note="Pentapeptide repeat protein Rfr32"
FT                   /id="PRO_0000434155"
FT   DISULFID        158..178
FT                   /evidence="ECO:0000269|PubMed:17075135,
FT                   ECO:0007744|PDB:2F3L"
FT   STRAND          48..51
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   STRAND          68..71
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   STRAND          88..91
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   STRAND          108..113
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   STRAND          128..133
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   HELIX           151..158
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   TURN            166..168
FT                   /evidence="ECO:0007829|PDB:2F3L"
FT   HELIX           172..175
FT                   /evidence="ECO:0007829|PDB:2F3L"
SQ   SEQUENCE   179 AA;  19215 MW;  15D1A2617EF0C368 CRC64;
     MVISRHDLGF IWMKYLLRLT IVVFSLLWLI TPTAYAASSS AVTGSSASYE DVKLIGEDFS
     GKSLTYAQFT NADLTDSNFS EADLRGAVFN GSALIGADLH GADLTNGLAY LTSFKGADLT
     NAVLTEAIMM RTKFDDAKIT GADFSLAVLD VYEVDKLCDR ADGVNPKTGV STRESLGCQ