RFRNP_AQUAE
ID RFRNP_AQUAE Reviewed; 192 AA.
AC O67035;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
DE Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
DE EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:29073018};
DE AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
GN OrderedLocusNames=aq_880;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND
RP MUTAGENESIS OF ASP-138; ASP-142; ASP-144 AND ASP-160.
RC STRAIN=VF5;
RX PubMed=29073018; DOI=10.1073/pnas.1707862114;
RA Nickel A.I., Waeber N.B., Goessringer M., Lechner M., Linne U., Toth U.,
RA Rossmanith W., Hartmann R.K.;
RT "Minimal and RNA-free RNase P in Aquifex aeolicus.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:11121-11126(2017).
CC -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC sequence from pre-tRNA to produce the mature 5'-terminus.
CC {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:29073018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01078, ECO:0000269|PubMed:29073018};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12 nM for pre-tRNA(Gly) {ECO:0000269|PubMed:29073018};
CC Note=kcat is 0.52 min(-1). {ECO:0000269|PubMed:29073018};
CC -!- SUBUNIT: Probably forms homotrimers. {ECO:0000269|PubMed:29073018}.
CC -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC Rule:MF_01078, ECO:0000305|PubMed:29073018}.
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DR EMBL; AE000657; AAC07003.1; -; Genomic_DNA.
DR PIR; H70375; H70375.
DR RefSeq; NP_213597.1; NC_000918.1.
DR RefSeq; WP_010880535.1; NC_000918.1.
DR PDB; 7F3E; EM; 3.62 A; A/B/C/D/E/F/G/H/I/J/K/L=1-192.
DR PDBsum; 7F3E; -.
DR AlphaFoldDB; O67035; -.
DR SMR; O67035; -.
DR STRING; 224324.aq_880; -.
DR EnsemblBacteria; AAC07003; AAC07003; aq_880.
DR KEGG; aae:aq_880; -.
DR PATRIC; fig|224324.8.peg.686; -.
DR eggNOG; COG1458; Bacteria.
DR HOGENOM; CLU_109672_0_0_0; -.
DR InParanoid; O67035; -.
DR OMA; YIIKKPP; -.
DR OrthoDB; 1359470at2; -.
DR BRENDA; 3.1.26.5; 396.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR014856; RNA_free_RNase_P.
DR PANTHER; PTHR41173; PTHR41173; 1.
DR Pfam; PF08745; PIN_5; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW tRNA processing.
FT CHAIN 1..192
FT /note="RNA-free ribonuclease P"
FT /id="PRO_0000136075"
FT MUTAGEN 138
FT /note="D->A: Abolishes activity."
FT /evidence="ECO:0000269|PubMed:29073018"
FT MUTAGEN 142
FT /note="D->A: Abolishes activity."
FT /evidence="ECO:0000269|PubMed:29073018"
FT MUTAGEN 144
FT /note="D->A: No change in activity."
FT /evidence="ECO:0000269|PubMed:29073018"
FT MUTAGEN 160
FT /note="D->A: Abolishes activity."
FT /evidence="ECO:0000269|PubMed:29073018"
SQ SEQUENCE 192 AA; 22532 MW; AC6A98DCC23E9600 CRC64;
MDVFVLDTSV FTNPEIYRTF EEDQRGAMET FIHLALNSRA EFYMPTSVYT EMRKIMDVGE
LWAEFEMVVK IRSPRRFQLT VPADFLYEFI EELRYRINKG LRIAEEHTRE ASGCEDVGKL
IARLREKYRE ALRQGILDSK EDVDVLLLAY ELDGVLVSAD EGLRTWADKI GIKLIDPKNF
KNILESLVRH RF
