ID   RFRNP_HALVD             Reviewed;         232 AA.
AC   D4GW50; A0A384KAR1; L9UTY9;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:31283101};
DE            EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:31283101};
DE   AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:31283101};
GN   OrderedLocusNames=HVO_1114 {ECO:0000312|EMBL:ADE04322.1};
GN   ORFNames=C498_13173 {ECO:0000312|EMBL:ELY28196.1};
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DS2 / DS70;
RX   PubMed=31283101; DOI=10.1002/iub.2122;
RA   Schwarz T.S., Waeber N.B., Feyh R., Weidenbach K., Schmitz R.A.,
RA   Marchfelder A., Hartmann R.K.;
RT   "Homologs of aquifex aeolicus protein-only RNase P are not the major RNase
RT   P activities in the archaea haloferax volcanii and methanosarcina mazei.";
RL   IUBMB Life 71:1109-1116(2019).
CC   -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC       sequence from pre-tRNA to produce the mature 5'-terminus.
CC       {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:31283101}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC         from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01078, ECO:0000269|PubMed:31283101};
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows no growth phenotype under
CC       standard conditions, temperature stress or salt stress.
CC       {ECO:0000269|PubMed:31283101}.
CC   -!- MISCELLANEOUS: The ease to obtain knockout of this gene and the lack of
CC       growth phenotypes upon gene deletion suggests that archaeal HARPs do
CC       not make a major contribution to global tRNA 5'-end maturation in
CC       archaea, but may well exert a specialised, yet unknown function in
CC       (t)RNA metabolism. {ECO:0000305|PubMed:31283101}.
CC   -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC       Rule:MF_01078}.
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DR   EMBL; CP001956; ADE04322.1; -; Genomic_DNA.
DR   EMBL; AOHU01000091; ELY28196.1; -; Genomic_DNA.
DR   RefSeq; WP_004043823.1; NZ_AOHU01000091.1.
DR   AlphaFoldDB; D4GW50; -.
DR   STRING; 309800.C498_13173; -.
DR   EnsemblBacteria; ADE04322; ADE04322; HVO_1114.
DR   EnsemblBacteria; ELY28196; ELY28196; C498_13173.
DR   GeneID; 8926228; -.
DR   KEGG; hvo:HVO_1114; -.
DR   PATRIC; fig|309800.29.peg.2529; -.
DR   eggNOG; arCOG00720; Archaea.
DR   HOGENOM; CLU_109672_0_0_2; -.
DR   OMA; YIIKKPP; -.
DR   OrthoDB; 68994at2157; -.
DR   Proteomes; UP000008243; Chromosome.
DR   Proteomes; UP000011532; Unassembled WGS sequence.
DR   GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR   InterPro; IPR014856; RNA_free_RNase_P.
DR   PANTHER; PTHR41173; PTHR41173; 1.
DR   Pfam; PF08745; PIN_5; 1.
DR   TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; tRNA processing.
FT   CHAIN           1..232
FT                   /note="RNA-free ribonuclease P"
FT                   /id="PRO_0000448616"
SQ   SEQUENCE   232 AA;  26431 MW;  D2B3DB7F215F1792 CRC64;
     MAEYPLKQRF VLDTSLFLSE EIRDDDETFE EGIERLLDTI AAARLDLNIS CYMPPSIAAE
     LEHILRERGV SEDVLGKLDT WVIKKHPDRY EVYIPAEVVY RFIDEMSDRV NRGLRVSEKA
     VRKAEESKQL SNGGSKLSDV DKVISDLRED YRGALRQGVL DSREDFDLLI LARELNAGVV
     TEDTGIISWA ADFGLRNLRG RAFPTLLEEY LVALDSPRPW TRGDDGVDPD RL