ID   RFRNP_METBU             Reviewed;         230 AA.
AC   Q12VF4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078};
DE   AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
GN   OrderedLocusNames=Mbur_1678;
OS   Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 /
OS   ACE-M).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M;
RX   PubMed=19404327; DOI=10.1038/ismej.2009.45;
RA   Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S.,
RA   De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., Ting L.,
RA   Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., Ivanova N.,
RA   Dalin E., Martinez M., Lapidus A., Hauser L., Land M., Thomas T.,
RA   Cavicchioli R.;
RT   "The genome sequence of the psychrophilic archaeon, Methanococcoides
RT   burtonii: the role of genome evolution in cold adaptation.";
RL   ISME J. 3:1012-1035(2009).
CC   -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC       sequence from pre-tRNA to produce the mature 5'-terminus.
CC       {ECO:0000255|HAMAP-Rule:MF_01078}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC         from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01078};
CC   -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC       Rule:MF_01078}.
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DR   EMBL; CP000300; ABE52572.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q12VF4; -.
DR   STRING; 259564.Mbur_1678; -.
DR   EnsemblBacteria; ABE52572; ABE52572; Mbur_1678.
DR   KEGG; mbu:Mbur_1678; -.
DR   HOGENOM; CLU_109672_0_0_2; -.
DR   OMA; YIIKKPP; -.
DR   Proteomes; UP000001979; Chromosome.
DR   GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR   InterPro; IPR014856; RNA_free_RNase_P.
DR   PANTHER; PTHR41173; PTHR41173; 1.
DR   Pfam; PF08745; PIN_5; 1.
DR   TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; tRNA processing.
FT   CHAIN           1..230
FT                   /note="RNA-free ribonuclease P"
FT                   /id="PRO_1000064799"
SQ   SEQUENCE   230 AA;  26459 MW;  4F1367D08517998B CRC64;
     MKQRFVLDTT ALTDLQTREM LEIEDLCQGM RAILDLIAES RLHLQISCYV PFPSVYKELH
     EFAKNNGCDD EIIAMIDTWL VKKTPDRHEV KIPSHIFHEY VSYMRERINK GMTVAEEAIR
     EASIQCLSIE SEIEDTRKVE DNTDREVIGP IVGKFRNKYR SALRYGILDS APDIDVLLLA
     KELDAAVVAS DYGIQKWAEQ LGVRFVPANT FPMMLKEYLR HTPSDGTHQK