RFRNP_METKA
ID RFRNP_METKA Reviewed; 228 AA.
AC Q8TW01;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
DE EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078};
DE AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
GN OrderedLocusNames=MK1237;
OS Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC Methanopyrus.
OX NCBI_TaxID=190192;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX PubMed=11930014; DOI=10.1073/pnas.032671499;
RA Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA Koonin E.V., Kozyavkin S.A.;
RT "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT monophyly of archaeal methanogens.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC sequence from pre-tRNA to produce the mature 5'-terminus.
CC {ECO:0000255|HAMAP-Rule:MF_01078}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01078};
CC -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC Rule:MF_01078}.
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DR EMBL; AE009439; AAM02450.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8TW01; -.
DR STRING; 190192.MK1237; -.
DR EnsemblBacteria; AAM02450; AAM02450; MK1237.
DR KEGG; mka:MK1237; -.
DR PATRIC; fig|190192.8.peg.1341; -.
DR HOGENOM; CLU_109672_0_0_2; -.
DR OMA; YIIKKPP; -.
DR Proteomes; UP000001826; Chromosome.
DR GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR InterPro; IPR014856; RNA_free_RNase_P.
DR PANTHER; PTHR41173; PTHR41173; 1.
DR Pfam; PF08745; PIN_5; 1.
DR TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Nuclease; Reference proteome; tRNA processing.
FT CHAIN 1..228
FT /note="RNA-free ribonuclease P"
FT /id="PRO_0000136080"
SQ SEQUENCE 228 AA; 26570 MW; 2AA73E88D1D352FA CRC64;
MTVYKQRFVL DTTAFTDRGV VDELGDGDLC EAVTRLLDLI GRARMELAIS CYIPYPTVYR
ELKGFLERNG CPELLSRIDT WIVKKTPNRY EVKVPARLFM AYVKDMRERL DRGRKRAEKA
IWEAALEAYE IMLREEADVP KERIIREVIG ETVRRFRRKY RQTVRHGTLD SAPDLDVLLL
AKELDAAVVA SDEGIERWAR ELGLRFMPAC SFPDMIEEYL RMSREVEG