ID   RFRNP_METMA             Reviewed;         247 AA.
AC   Q8PV94;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:31283101};
DE            EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:31283101};
DE   AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:31283101};
GN   OrderedLocusNames=MM_2077;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=31283101; DOI=10.1002/iub.2122;
RA   Schwarz T.S., Waeber N.B., Feyh R., Weidenbach K., Schmitz R.A.,
RA   Marchfelder A., Hartmann R.K.;
RT   "Homologs of aquifex aeolicus protein-only RNase P are not the major RNase
RT   P activities in the archaea haloferax volcanii and methanosarcina mazei.";
RL   IUBMB Life 71:1109-1116(2019).
CC   -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC       sequence from pre-tRNA to produce the mature 5'-terminus.
CC       {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:31283101}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC         from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01078, ECO:0000269|PubMed:31283101};
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows no growth phenotype under
CC       standard conditions or nitrogen deficiency.
CC       {ECO:0000269|PubMed:31283101}.
CC   -!- MISCELLANEOUS: The ease to obtain knockout of this gene and the lack of
CC       growth phenotypes upon gene deletion suggests that archaeal HARPs do
CC       not make a major contribution to global tRNA 5'-end maturation in
CC       archaea, but may well exert a specialised, yet unknown function in
CC       (t)RNA metabolism. {ECO:0000305|PubMed:31283101}.
CC   -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC       Rule:MF_01078}.
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DR   EMBL; AE008384; AAM31773.1; -; Genomic_DNA.
DR   RefSeq; WP_011034009.1; NC_003901.1.
DR   AlphaFoldDB; Q8PV94; -.
DR   STRING; 192952.MM_2077; -.
DR   EnsemblBacteria; AAM31773; AAM31773; MM_2077.
DR   GeneID; 44088294; -.
DR   GeneID; 66136447; -.
DR   KEGG; mma:MM_2077; -.
DR   PATRIC; fig|192952.21.peg.2384; -.
DR   eggNOG; arCOG00720; Archaea.
DR   HOGENOM; CLU_109672_0_0_2; -.
DR   OMA; YIIKKPP; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR   InterPro; IPR014856; RNA_free_RNase_P.
DR   PANTHER; PTHR41173; PTHR41173; 1.
DR   Pfam; PF08745; PIN_5; 1.
DR   TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; tRNA processing.
FT   CHAIN           1..247
FT                   /note="RNA-free ribonuclease P"
FT                   /id="PRO_0000136081"
SQ   SEQUENCE   247 AA;  28672 MW;  062EA19F6C19A498 CRC64;
     MLKQRFVLDT TALTDLQTRE VMGYTSLCEG MKTILDLIAE ARLHFGISCY VPYPSVYKEM
     YEFASRNGCD REVVAKIDTW LVKKSPDRYR VDVTSQIFHE YVSYMRERIN RGMGVAEDAI
     WEAATECLFM ENPQNKKKEY REEVEREVIG GIIGKFRNKY RAALRYGILD SAPDIDVLIL
     AKELDAAVIA SDYGIEKWAE QLGVRFVPAN TFPMMIKEYL RHGPEVVKEQ EDEDRKRIDY
     SDDADFI