RFRNP_METTH
ID RFRNP_METTH Reviewed; 220 AA.
AC O26345;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
DE Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
DE EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:29073018};
DE AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000303|PubMed:29073018};
GN OrderedLocusNames=MTH_243;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=29073018; DOI=10.1073/pnas.1707862114;
RA Nickel A.I., Waeber N.B., Goessringer M., Lechner M., Linne U., Toth U.,
RA Rossmanith W., Hartmann R.K.;
RT "Minimal and RNA-free RNase P in Aquifex aeolicus.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:11121-11126(2017).
CC -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC sequence from pre-tRNA to produce the mature 5'-terminus.
CC {ECO:0000255|HAMAP-Rule:MF_01078, ECO:0000269|PubMed:29073018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01078, ECO:0000269|PubMed:29073018};
CC -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC Rule:MF_01078, ECO:0000305|PubMed:29073018}.
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DR EMBL; AE000666; AAB84749.1; -; Genomic_DNA.
DR PIR; D69130; D69130.
DR RefSeq; WP_010875882.1; NC_000916.1.
DR AlphaFoldDB; O26345; -.
DR STRING; 187420.MTH_243; -.
DR EnsemblBacteria; AAB84749; AAB84749; MTH_243.
DR GeneID; 1470204; -.
DR KEGG; mth:MTH_243; -.
DR PATRIC; fig|187420.15.peg.212; -.
DR HOGENOM; CLU_109672_0_0_2; -.
DR OMA; YIIKKPP; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR InterPro; IPR014856; RNA_free_RNase_P.
DR PANTHER; PTHR41173; PTHR41173; 1.
DR Pfam; PF08745; PIN_5; 1.
DR TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE 1: Evidence at protein level;
KW Endonuclease; Hydrolase; Nuclease; Reference proteome; tRNA processing.
FT CHAIN 1..220
FT /note="RNA-free ribonuclease P"
FT /id="PRO_0000136083"
SQ SEQUENCE 220 AA; 25461 MW; 8D5D01DA37ED4CF4 CRC64;
MLAKQRFVLD TTAFTDNQLR ELLGDGDLNL SVDRMLDLIA RSRIKLNISC HMPPITYKEF
TDYMTRYDCP EETLIKAETW IVKKTPNRYD TQIPSQIFYE YVHDIRERMN KGLRISETLL
WEAGIQSIIM ASRDVKKTEI ESEVLGKAIK DLRKKYRSAL RKGTLDSAPD LDVLLLAKEL
GAGVVAADDG IRVWAERLGL RFLNATSFPK MLKEYLKYYE
