ID   RFRNP_PYRAB             Reviewed;         204 AA.
AC   Q9V1N4; G8ZI28;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078};
DE   AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
GN   OrderedLocusNames=PYRAB03930; ORFNames=PAB0263;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
CC   -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC       sequence from pre-tRNA to produce the mature 5'-terminus.
CC       {ECO:0000255|HAMAP-Rule:MF_01078}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC         from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01078};
CC   -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC       Rule:MF_01078}.
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DR   EMBL; AJ248284; CAB49315.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE69771.1; -; Genomic_DNA.
DR   PIR; D75154; D75154.
DR   RefSeq; WP_048146546.1; NC_000868.1.
DR   AlphaFoldDB; Q9V1N4; -.
DR   SMR; Q9V1N4; -.
DR   STRING; 272844.PAB0263; -.
DR   EnsemblBacteria; CAB49315; CAB49315; PAB0263.
DR   GeneID; 1495284; -.
DR   KEGG; pab:PAB0263; -.
DR   PATRIC; fig|272844.11.peg.414; -.
DR   eggNOG; arCOG00720; Archaea.
DR   HOGENOM; CLU_109672_0_0_2; -.
DR   OMA; YIIKKPP; -.
DR   OrthoDB; 68994at2157; -.
DR   PhylomeDB; Q9V1N4; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR014856; RNA_free_RNase_P.
DR   PANTHER; PTHR41173; PTHR41173; 1.
DR   Pfam; PF08745; PIN_5; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; tRNA processing.
FT   CHAIN           1..204
FT                   /note="RNA-free ribonuclease P"
FT                   /id="PRO_0000136084"
SQ   SEQUENCE   204 AA;  23890 MW;  A265C470637FFED9 CRC64;
     MIRFVLDTSI FVNPDVRKKF GETPTKAMKT FLKYAESLFG HVEFYMPPGI YREVMHFVEE
     EEVSPDIELY IIKKPPNVHD IKIPAFVVYE LIEDIRRRVD KGLRVAEKAV RESVIDTSNV
     DKIIQKLRRN YRKALREGIL DSKEDFELIL LAKELDGIIV SADVGILTWA EKMGIKWVDA
     FKFKEVLEEL VEKLKRSESE KERK