ID   RFRNP_PYRHO             Reviewed;         204 AA.
AC   O59517;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   09-SEP-2003, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=RNA-free ribonuclease P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            Short=RNA-free RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
DE            EC=3.1.26.5 {ECO:0000255|HAMAP-Rule:MF_01078};
DE   AltName: Full=Protein-only RNase P {ECO:0000255|HAMAP-Rule:MF_01078};
GN   OrderedLocusNames=PH1839;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: RNA-free RNase P that catalyzes the removal of the 5'-leader
CC       sequence from pre-tRNA to produce the mature 5'-terminus.
CC       {ECO:0000255|HAMAP-Rule:MF_01078}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides
CC         from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01078};
CC   -!- SIMILARITY: Belongs to the HARP family. {ECO:0000255|HAMAP-
CC       Rule:MF_01078}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA30959.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000001; BAA30959.1; ALT_INIT; Genomic_DNA.
DR   PIR; H71195; H71195.
DR   RefSeq; WP_048053492.1; NC_000961.1.
DR   AlphaFoldDB; O59517; -.
DR   SMR; O59517; -.
DR   STRING; 70601.3258276; -.
DR   DNASU; 1442680; -.
DR   EnsemblBacteria; BAA30959; BAA30959; BAA30959.
DR   GeneID; 1442680; -.
DR   KEGG; pho:PH1839; -.
DR   eggNOG; arCOG00720; Archaea.
DR   OMA; YIIKKPP; -.
DR   OrthoDB; 68994at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001682; P:tRNA 5'-leader removal; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01078; RNA_free_RNase_P; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR014856; RNA_free_RNase_P.
DR   PANTHER; PTHR41173; PTHR41173; 1.
DR   Pfam; PF08745; PIN_5; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR03875; RNA_lig_partner; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; tRNA processing.
FT   CHAIN           1..204
FT                   /note="RNA-free ribonuclease P"
FT                   /id="PRO_0000136086"
SQ   SEQUENCE   204 AA;  23896 MW;  DDB318BEBD486436 CRC64;
     MIKFVLDTSI FVNPDVRKKF GETPTEAMKT FLHYAENLFG KVEFYMPPGI YRELMHFVEE
     EEVSPDIELY IIKKPPNVHD IKIPAFVVYE LIEDIRRRVD KGLRVAEKAV RESVIDTSNV
     DKIIQKLRRN YRKALREGIL DSKEDFELIL LAKEIDGIIV SADVGILTWA EKMGIKWVDA
     FKFKEVLSEL VEKFKRSESE KERK