RFS1_ARATH
ID RFS1_ARATH Reviewed; 754 AA.
AC Q84VX0; Q9LFZ7;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Probable galactinol--sucrose galactosyltransferase 1;
DE EC=2.4.1.82;
DE AltName: Full=Protein SEED IMBIBITION 1;
DE AltName: Full=Raffinose synthase 1;
GN Name=RFS1; Synonyms=RS1, SIP1; OrderedLocusNames=At1g55740;
GN ORFNames=F20N2.12, F20N2.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC seeds, roots and tubers (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC -!- INDUCTION: Not induced by oxidative stress.
CC {ECO:0000269|PubMed:18502973}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79504.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g55730 and At1g55740.; Evidence={ECO:0000305};
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DR EMBL; AC002328; AAF79504.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33292.1; -; Genomic_DNA.
DR EMBL; BT004640; AAO42886.1; -; mRNA.
DR EMBL; AK227977; BAE99943.1; -; mRNA.
DR PIR; C96599; C96599.
DR RefSeq; NP_175970.1; NM_104450.4.
DR AlphaFoldDB; Q84VX0; -.
DR BioGRID; 27248; 1.
DR IntAct; Q84VX0; 1.
DR STRING; 3702.AT1G55740.1; -.
DR CAZy; GH36; Glycoside Hydrolase Family 36.
DR PaxDb; Q84VX0; -.
DR PRIDE; Q84VX0; -.
DR ProteomicsDB; 236923; -.
DR EnsemblPlants; AT1G55740.1; AT1G55740.1; AT1G55740.
DR GeneID; 842023; -.
DR Gramene; AT1G55740.1; AT1G55740.1; AT1G55740.
DR KEGG; ath:AT1G55740; -.
DR Araport; AT1G55740; -.
DR TAIR; locus:2020452; AT1G55740.
DR eggNOG; ENOG502QPVE; Eukaryota.
DR HOGENOM; CLU_007066_0_0_1; -.
DR InParanoid; Q84VX0; -.
DR OMA; SGMEHYE; -.
DR OrthoDB; 142079at2759; -.
DR PhylomeDB; Q84VX0; -.
DR BioCyc; ARA:AT1G55740-MON; -.
DR BRENDA; 2.4.1.82; 399.
DR PRO; PR:Q84VX0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84VX0; baseline and differential.
DR Genevisible; Q84VX0; AT.
DR GO; GO:0047274; F:galactinol-sucrose galactosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR008811; Glycosyl_hydrolases_36.
DR PANTHER; PTHR31268; PTHR31268; 1.
DR Pfam; PF05691; Raffinose_syn; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..754
FT /note="Probable galactinol--sucrose galactosyltransferase
FT 1"
FT /id="PRO_0000270157"
SQ SEQUENCE 754 AA; 83477 MW; C57DB5076DADA8AD CRC64;
MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD QTGSHRVFSL
GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE ANQGSDLGGR DQSSSYVVFL
PILEGDFRAV LQGNEANELE ICLESGDPTV DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ
HLQTFSHRER KKMPDMLNWF GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW
QSVGMDETSV EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN
NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC LESITKNGLG
LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG HGGRVKLAKK YHQALEASIS
RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA SDDFWPRDPA SHTIHIASVA YNTLFLGEFM
QPDWDMFHSL HPMAEYHAAA RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR
PTSDCFFSDP VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC
VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE VFTVVPVKEF
SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR GSGLVGVYSS VRRPRSVTVD
SDDVEYRYEP ESGLVTFTLG VPEKELYLWD VVIQ
