RFS2_ARATH
ID RFS2_ARATH Reviewed; 773 AA.
AC Q94A08; Q0WWI0; Q3EAI2; Q3EAI3; Q9SCM1;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Probable galactinol--sucrose galactosyltransferase 2;
DE EC=2.4.1.82;
DE AltName: Full=Protein SEED IMBIBITION 2;
DE AltName: Full=Raffinose synthase 2;
GN Name=RFS2; Synonyms=RS2, SIP2; OrderedLocusNames=At3g57520;
GN ORFNames=T8H10.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 435-773 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC seeds, roots and tubers (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q94A08-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94A08-2; Sequence=VSP_038414, VSP_038415;
CC Name=3;
CC IsoId=Q94A08-3; Sequence=VSP_038412, VSP_038413;
CC -!- INDUCTION: By oxidative stress. {ECO:0000269|PubMed:18502973}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC {ECO:0000305}.
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DR EMBL; AL133248; CAB66109.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79665.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79667.1; -; Genomic_DNA.
DR EMBL; AY050772; AAK92707.1; -; mRNA.
DR EMBL; AK226370; BAE98518.1; -; mRNA.
DR PIR; T46188; T46188.
DR RefSeq; NP_191311.1; NM_115612.3. [Q94A08-1]
DR RefSeq; NP_850715.1; NM_180384.1. [Q94A08-2]
DR AlphaFoldDB; Q94A08; -.
DR BioGRID; 10235; 2.
DR STRING; 3702.AT3G57520.1; -.
DR CAZy; GH36; Glycoside Hydrolase Family 36.
DR PaxDb; Q94A08; -.
DR PRIDE; Q94A08; -.
DR ProteomicsDB; 236235; -. [Q94A08-1]
DR EnsemblPlants; AT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
DR EnsemblPlants; AT3G57520.2; AT3G57520.2; AT3G57520. [Q94A08-2]
DR GeneID; 824919; -.
DR Gramene; AT3G57520.1; AT3G57520.1; AT3G57520. [Q94A08-1]
DR Gramene; AT3G57520.2; AT3G57520.2; AT3G57520. [Q94A08-2]
DR KEGG; ath:AT3G57520; -.
DR Araport; AT3G57520; -.
DR TAIR; locus:2103488; AT3G57520.
DR eggNOG; ENOG502QPVE; Eukaryota.
DR HOGENOM; CLU_007066_0_0_1; -.
DR InParanoid; Q94A08; -.
DR OMA; EAVYVVM; -.
DR OrthoDB; 142079at2759; -.
DR PhylomeDB; Q94A08; -.
DR BioCyc; ARA:AT3G57520-MON; -.
DR BioCyc; MetaCyc:AT3G57520-MON; -.
DR BRENDA; 2.4.1.82; 399.
DR PRO; PR:Q94A08; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q94A08; baseline and differential.
DR Genevisible; Q94A08; AT.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0052692; F:raffinose alpha-galactosidase activity; IDA:TAIR.
DR GO; GO:0034484; P:raffinose catabolic process; IDA:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR008811; Glycosyl_hydrolases_36.
DR PANTHER; PTHR31268; PTHR31268; 1.
DR Pfam; PF05691; Raffinose_syn; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carbohydrate metabolism; Glycosyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..773
FT /note="Probable galactinol--sucrose galactosyltransferase
FT 2"
FT /id="PRO_0000389256"
FT VAR_SEQ 552..565
FT /note="SLLKIWNMNKFTGI -> RCWLVQGNEEEPD (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038412"
FT VAR_SEQ 566..773
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038413"
FT VAR_SEQ 655..656
FT /note="EI -> DL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038414"
FT VAR_SEQ 657..773
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038415"
FT CONFLICT 255
FT /note="Q -> R (in Ref. 3; AAK92707)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 773 AA; 85143 MW; 0852F9E67952C8D3 CRC64;
MTITSNISVQ NDNLVVQGKT ILTKIPDNII LTPVTGNGFV SGSFIGATFE QSKSLHVFPI
GVLEGLRFMC CFRFKLWWMT QRMGSCGKDI PLETQFMLLE SKDEVEGNGD DAPTVYTVFL
PLLEGQFRAV LQGNEKNEIE ICFESGDKAV ETSQGTHLVY VHAGTNPFEV IRQSVKAVER
HMQTFHHREK KKLPSFLDWF GWCTWDAFYT DVTAEGVDEG LKSLSEGGTP PKFLIIDDGW
QQIENKEKDE NCVVQEGAQF ATRLVGIKEN AKFQKSDQKD TQVSGLKSVV DNAKQRHNVK
QVYAWHALAG YWGGVKPAAS GMEHYDSALA YPVQSPGVLG NQPDIVMDSL AVHGLGLVNP
KKVFNFYNEL HSYLASCGID GVKVDVQNII ETLGAGLGGR VSLTRSYQQA LEASIARNFT
DNGCISCMCH NTDGLYSAKQ TAIVRASDDF YPRDPASHTI HIASVAYNSL FLGEFMQPDW
DMFHSLHPTA EYHAAARAVG GCAIYVSDKP GNHNFDLLRK LVLPDGSVLR AKLPGRPTRD
CLFADPARDG ISLLKIWNMN KFTGIVGVFN CQGAGWCKET KKNQIHDTSP GTLTGSIRAD
DADLISQVAG EDWSGDSIVY AYRSGEVVRL PKGASIPLTL KVLEYELFHI SPLKEITENI
SFAPIGLVDM FNSSGAIESI DINHVTDKNP EFFDGEISSA SPALSDNRSP TALVSVSVRG
CGRFGAYSSQ RPLKCAVEST ETDFTYDAEV GLVTLNLPVT REEMFRWHVE ILV
