ID   RFS4_ARATH              Reviewed;         876 AA.
AC   Q9SYJ4; Q0WPF3;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 3.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Probable galactinol--sucrose galactosyltransferase 4;
DE            EC=2.4.1.82;
DE   AltName: Full=Raffinose synthase 4;
GN   Name=RFS4; Synonyms=RS4; OrderedLocusNames=At4g01970; ORFNames=T7B11.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18502973; DOI=10.1104/pp.108.122465;
RA   Nishizawa A., Yabuta Y., Shigeoka S.;
RT   "Galactinol and raffinose constitute a novel function to protect plants
RT   from oxidative damage.";
RL   Plant Physiol. 147:1251-1263(2008).
CC   -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC       Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC       seeds, roots and tubers (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC         inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC         ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC   -!- INDUCTION: By oxidative stress. {ECO:0000269|PubMed:18502973}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD22659.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB80690.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007138; AAD22659.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161493; CAB80690.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE82106.1; -; Genomic_DNA.
DR   EMBL; AK229121; BAF00996.1; -; mRNA.
DR   PIR; C85025; C85025.
DR   RefSeq; NP_192106.3; NM_116428.5.
DR   AlphaFoldDB; Q9SYJ4; -.
DR   STRING; 3702.AT4G01970.1; -.
DR   CAZy; GH36; Glycoside Hydrolase Family 36.
DR   PaxDb; Q9SYJ4; -.
DR   PRIDE; Q9SYJ4; -.
DR   ProteomicsDB; 236937; -.
DR   EnsemblPlants; AT4G01970.1; AT4G01970.1; AT4G01970.
DR   GeneID; 828186; -.
DR   Gramene; AT4G01970.1; AT4G01970.1; AT4G01970.
DR   KEGG; ath:AT4G01970; -.
DR   Araport; AT4G01970; -.
DR   TAIR; locus:2141425; AT4G01970.
DR   eggNOG; ENOG502QTKB; Eukaryota.
DR   HOGENOM; CLU_007066_0_0_1; -.
DR   InParanoid; Q9SYJ4; -.
DR   BRENDA; 2.4.1.82; 399.
DR   PRO; PR:Q9SYJ4; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9SYJ4; baseline and differential.
DR   Genevisible; Q9SYJ4; AT.
DR   GO; GO:0047268; F:galactinol-raffinose galactosyltransferase activity; IDA:TAIR.
DR   GO; GO:0047274; F:galactinol-sucrose galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR008811; Glycosyl_hydrolases_36.
DR   PANTHER; PTHR31268; PTHR31268; 1.
DR   Pfam; PF05691; Raffinose_syn; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glycosyltransferase; Phosphoprotein;
KW   Reference proteome; Transferase.
FT   CHAIN           1..876
FT                   /note="Probable galactinol--sucrose galactosyltransferase
FT                   4"
FT                   /id="PRO_0000389257"
FT   MOD_RES         7
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FND9"
FT   MOD_RES         9
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FND9"
FT   CONFLICT        792
FT                   /note="A -> V (in Ref. 3; BAF00996)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   876 AA;  98012 MW;  9155965DD4EF0FAF CRC64;
     MAPLHESLSS INDVIESKPL FVPITKPILQ PNSFNLSEGS LCAKDSTPIL FDVPQNVTFT
     PFSSHSISTD APLPILLRVQ ANAHKGGFLG FTKESPSDRL TNSLGRFEDR EFLSLFRFKM
     WWSTAWIGKS GSDLQAETQW VMLKIPEIDS YVAIIPTIEG AFRASLTPGE KGNVLICAES
     GSTKVKESSF KSIAYIHICD NPYNLMKEAF SALRVHMNTF KLLEEKKLPK IVDKFGWCTW
     DACYLTVDPA TIWTGVKEFE DGGVCPKFVI IDDGWQSINF DGDELDKDAE NLVLGGEQMT
     ARLTSFKECK KFRNYKGGSF ITSDASHFNP LKPKMLIYKA TERIQAIILR RKLVKESGEQ
     DLTELDEKIK ILSEELNAMF DEVEKEESLG SDDVSGSGMA AFTKDLRLRF KSLDDIYVWH
     ALCGAWNGVR PETMMDLKAK VAPFELSPSL GATMADLAVD KVVEAGIGLV HPSKAHEFYD
     SMHSYLASVG VTGAKIDVFQ TLESLAEEHG GRVELAKAYY DGLTESMIKN FNGTDVIASM
     QQCNEFFFLA TKQISIGRVG DDFWWQDPYG DPQGVYWLQG VHMIHCSYNS IWMGQMIQPD
     WDMFQSDHVC AEYHAASRAI CGGPVYLSDH LGKASHNFDL IKKLAFFDGT IPRCVHYALP
     TRDSLFKNPL FDKESILKIF NFNKFGGVIG TFNCQGAGWS PEEHRFKGYK ECYTTVSGTV
     HVSDIEWDQN PEAAGSQVTY TGDYLVYKQQ SEEILFMNSK SEAMKITLEP SAFDLLSFVP
     VTELVSSGVR FAPLGLINMF NCVGTVQDMK VTGDNSIRVD VKGEGRFMAY SSSAPVKCYL
     NDKEAEFKWE EETGKLSFFV PWVEESGGIS HLSFTF