RFS5_ARATH
ID RFS5_ARATH Reviewed; 783 AA.
AC Q9FND9;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable galactinol--sucrose galactosyltransferase 5;
DE EC=2.4.1.82;
DE AltName: Full=Protein SEED IMBIBITION 1-LIKE;
DE AltName: Full=Raffinose synthase 5;
GN Name=RFS5; Synonyms=RS5; OrderedLocusNames=At5g40390;
GN ORFNames=MPO12.100, MPO12.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP DISRUPTION PHENOTYPE.
RA Anderson C.M., Kohorn B.D.;
RT "Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and
RT drought tolerance.";
RL J. Plant Physiol. 158:1215-1219(2001).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=15474032; DOI=10.1016/j.febslet.2004.09.006;
RA Zuther E., Buechel K., Hundertmark M., Stitt M., Hincha D.K., Heyer A.G.;
RT "The role of raffinose in the cold acclimation response of Arabidopsis
RT thaliana.";
RL FEBS Lett. 576:169-173(2004).
RN [6]
RP INDUCTION BY COLD.
RX PubMed=17461790; DOI=10.1111/j.1365-313x.2007.03100.x;
RA Kaplan F., Kopka J., Sung D.Y., Zhao W., Popp M., Porat R., Guy C.L.;
RT "Transcript and metabolite profiling during cold acclimation of Arabidopsis
RT reveals an intricate relationship of cold-regulated gene expression with
RT modifications in metabolite content.";
RL Plant J. 50:967-981(2007).
RN [7]
RP INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-11, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC seeds, roots and tubers (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC -!- INDUCTION: By oxidative stress and cold treatment.
CC {ECO:0000269|PubMed:17461790, ECO:0000269|PubMed:18502973}.
CC -!- DISRUPTION PHENOTYPE: Reduced drought tolerance, but no effect on
CC freezing tolerance or on the ability to cold acclimation.
CC {ECO:0000269|PubMed:15474032, ECO:0000269|Ref.4}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC {ECO:0000305}.
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DR EMBL; AB006702; BAB11595.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94542.1; -; Genomic_DNA.
DR EMBL; AY062781; AAL32859.1; -; mRNA.
DR EMBL; AY081645; AAM10207.1; -; mRNA.
DR RefSeq; NP_198855.1; NM_123403.4.
DR AlphaFoldDB; Q9FND9; -.
DR BioGRID; 19288; 1.
DR STRING; 3702.AT5G40390.1; -.
DR CAZy; GH36; Glycoside Hydrolase Family 36.
DR iPTMnet; Q9FND9; -.
DR PaxDb; Q9FND9; -.
DR PRIDE; Q9FND9; -.
DR ProteomicsDB; 236262; -.
DR EnsemblPlants; AT5G40390.1; AT5G40390.1; AT5G40390.
DR GeneID; 834037; -.
DR Gramene; AT5G40390.1; AT5G40390.1; AT5G40390.
DR KEGG; ath:AT5G40390; -.
DR Araport; AT5G40390; -.
DR TAIR; locus:2170528; AT5G40390.
DR eggNOG; ENOG502QTKB; Eukaryota.
DR HOGENOM; CLU_007066_0_0_1; -.
DR InParanoid; Q9FND9; -.
DR OMA; NQCFSEC; -.
DR OrthoDB; 142079at2759; -.
DR PhylomeDB; Q9FND9; -.
DR BioCyc; ARA:AT5G40390-MON; -.
DR BRENDA; 2.4.1.82; 399.
DR PRO; PR:Q9FND9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FND9; baseline and differential.
DR Genevisible; Q9FND9; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0047274; F:galactinol-sucrose galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR008811; Glycosyl_hydrolases_36.
DR PANTHER; PTHR31268; PTHR31268; 1.
DR Pfam; PF05691; Raffinose_syn; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Glycosyltransferase; Phosphoprotein;
KW Reference proteome; Transferase.
FT CHAIN 1..783
FT /note="Probable galactinol--sucrose galactosyltransferase
FT 5"
FT /id="PRO_0000389258"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 783 AA; 86237 MW; 3C37D1D7871888AC CRC64;
MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP YLVDKDGVPL
DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV WWTTHWVGSN GRDIENETQI
IILDQSGSDS GPGSGSGRPY VLLLPLLEGS FRSSFQSGED DDVAVCVESG STEVTGSEFR
QIVYVHAGDD PFKLVKDAMK VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG
VHKGVKCLVD GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK
FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP ALPPSTIIRP
ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS HLQNAGIDGV KVDVIHILEM
LCQKYGGRVD LAKAYFKALT SSVNKHFNGN GVIASMEHCN DFMFLGTEAI SLGRVGDDFW
CTDPSGDPNG TFWLQGCHMV HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP
IYISDCVGKH DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT
GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI ANVEEFALFL
SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV RFAPIGLVNM LNTSGAIRSL
VYNDESVEVG VFGAGEFRVY ASKKPVSCLI DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ
YLF
