ID   RFS_PEA                 Reviewed;         798 AA.
AC   Q8VWN6;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Galactinol--sucrose galactosyltransferase;
DE            EC=2.4.1.82;
DE   AltName: Full=Raffinose synthase;
GN   Name=RFS;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ACTIVITY
RP   REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=cv. Kelvedon Wonder; TISSUE=Seed;
RX   PubMed=12244450; DOI=10.1007/s00425-002-0804-7;
RA   Peterbauer T., Mach L., Mucha J., Richter A.;
RT   "Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose
RT   synthase, partial purification of the enzyme from maturing seeds, and
RT   steady-state kinetic analysis of raffinose synthesis.";
RL   Planta 215:839-846(2002).
CC   -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC       Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC       seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as
CC       acceptors. May also act as a glycoside hydrolase.
CC       {ECO:0000269|PubMed:12244450}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC         inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC         ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC         Evidence={ECO:0000269|PubMed:12244450};
CC   -!- ACTIVITY REGULATION: Inhibited by 1-deoxygalactonojirimycin. Not
CC       inhibited by stachyose. Strong inhibition of the hydrolytic activity by
CC       sucrose. {ECO:0000269|PubMed:12244450}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.3 mM for galactinol for the raffinose synthesis activity
CC         {ECO:0000269|PubMed:12244450};
CC         KM=22.9 mM for sucrose for the raffinose synthesis activity
CC         {ECO:0000269|PubMed:12244450};
CC         KM=1.0 mM for galactinol for the galactinol hydrolysis activity
CC         {ECO:0000269|PubMed:12244450};
CC         Vmax=199.2 pmol/sec/mg enzyme for the raffinose synthesis activity
CC         {ECO:0000269|PubMed:12244450};
CC         Vmax=27.3 pmol/sec/mg enzyme for the galactinol hydrolysis activity
CC         {ECO:0000269|PubMed:12244450};
CC         Note=partially purified enzyme.;
CC       pH dependence:
CC         Optimum pH is 7.0. {ECO:0000269|PubMed:12244450};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ426475; CAD20127.2; -; mRNA.
DR   AlphaFoldDB; Q8VWN6; -.
DR   CAZy; GH36; Glycoside Hydrolase Family 36.
DR   PRIDE; Q8VWN6; -.
DR   EnsemblPlants; Psat0s2597g0160.1; Psat0s2597g0160.1.cds; Psat0s2597g0160.
DR   Gramene; Psat0s2597g0160.1; Psat0s2597g0160.1.cds; Psat0s2597g0160.
DR   BioCyc; MetaCyc:MON-12496; -.
DR   BRENDA; 2.4.1.82; 4872.
DR   GO; GO:0047274; F:galactinol-sucrose galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR008811; Glycosyl_hydrolases_36.
DR   PANTHER; PTHR31268; PTHR31268; 1.
DR   Pfam; PF05691; Raffinose_syn; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Glycosyltransferase; Transferase.
FT   CHAIN           1..798
FT                   /note="Galactinol--sucrose galactosyltransferase"
FT                   /id="PRO_0000389254"
SQ   SEQUENCE   798 AA;  88717 MW;  8D3F3ED5BF8617B7 CRC64;
     MAPPSITKTA TQQDVISTVD IGNSPLLSIS LDQSRNFLVN GHPFLTQVPP NITTTTTSTP
     SPFLDFKSNK DTIANNNNTL QQQGCFVGFN TTEAKSHHVV PLGKLKGIKF TSIFRFKVWW
     TTHWVGTNGH ELQHETQILI LDKNISLGRP YVLLLPILEN SFRTSLQPGL NDYVDMSVES
     GSTHVTGSTF KACLYLHLSN DPYRLVKEAV KVIQTKLGTF KTLEEKTPPS IIEKFGWCTW
     DAFYLKVHPK GVWEGVKALT DGGCPPGFVI IDDGWQSISH DDDDPVTERD GMNRTSAGEQ
     MPCRLIKYEE NYKFREYENG DNGGKKGLVG FVRDLKEEFR SVESVYVWHA LCGYWGGVRP
     KVCGMPEAKV VVPKLSPGVK MTMEDLAVDK IVENGVGLVP PNLAQEMFDG IHSHLESAGI
     DGVKVDVIHL LELLSEEYGG RVELAKAYYK ALTSSVNKHF KGNGVIASME HCNDFFLLGT
     EAISLGRVGD DFWCCDPSGD PNGTYWLQGC HMVHCAYNSL WMGNFIHPDW DMFQSTHPCA
     EFHAASRAIS GGPVYVSDCV GNHNFKLLKS FVLPDGSILR CQHYALPTRD CLFEDPLHNG
     KTMLKIWNLN KYAGVLGLFN CQGGGWCPET RRNKSASEFS HAVTCYASPE DIEWCNGKTP
     MDIKGVDVFA VYFFKEKKLS LMKCSDRLEV SLEPFSFELM TVSPLKVFSK RLIQFAPIGL
     VNMLNSGGAV QSLEFDDSAS LVKIGVRGCG ELSVFASEKP VCCKIDGVSV EFDYEDKMVR
     VQILWPGSST LSLVEFLF