RFT1_MOUSE
ID RFT1_MOUSE Reviewed; 541 AA.
AC Q8C3B8; B2RUN9;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein RFT1 homolog;
GN Name=Rft1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in N-linked oligosaccharide assembly. May
CC participate in the translocation of oligosaccharide from the
CC cytoplasmic side to the lumenal side of the endoplasmic reticulum
CC membrane (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C3B8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C3B8-2; Sequence=VSP_029509, VSP_029510;
CC -!- SIMILARITY: Belongs to the RFT1 family. {ECO:0000305}.
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DR EMBL; AK086390; BAC39658.1; -; mRNA.
DR EMBL; AC154436; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154646; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC116368; AAI16369.1; -; mRNA.
DR EMBL; BC116369; AAI16370.1; -; mRNA.
DR EMBL; BC141275; AAI41276.1; -; mRNA.
DR CCDS; CCDS26896.1; -. [Q8C3B8-1]
DR RefSeq; NP_808483.2; NM_177815.3. [Q8C3B8-1]
DR AlphaFoldDB; Q8C3B8; -.
DR SMR; Q8C3B8; -.
DR BioGRID; 236591; 2.
DR STRING; 10090.ENSMUSP00000064153; -.
DR iPTMnet; Q8C3B8; -.
DR PhosphoSitePlus; Q8C3B8; -.
DR MaxQB; Q8C3B8; -.
DR PaxDb; Q8C3B8; -.
DR PRIDE; Q8C3B8; -.
DR ProteomicsDB; 255011; -. [Q8C3B8-1]
DR ProteomicsDB; 255012; -. [Q8C3B8-2]
DR Ensembl; ENSMUST00000064230; ENSMUSP00000064153; ENSMUSG00000052395. [Q8C3B8-1]
DR GeneID; 328370; -.
DR KEGG; mmu:328370; -.
DR UCSC; uc007svm.1; mouse. [Q8C3B8-2]
DR UCSC; uc007svn.1; mouse. [Q8C3B8-1]
DR CTD; 91869; -.
DR MGI; MGI:3607791; Rft1.
DR VEuPathDB; HostDB:ENSMUSG00000052395; -.
DR eggNOG; KOG2864; Eukaryota.
DR GeneTree; ENSGT00390000011390; -.
DR HOGENOM; CLU_023360_5_0_1; -.
DR InParanoid; Q8C3B8; -.
DR OMA; WPGKLFG; -.
DR OrthoDB; 582756at2759; -.
DR PhylomeDB; Q8C3B8; -.
DR TreeFam; TF313129; -.
DR BioGRID-ORCS; 328370; 22 hits in 70 CRISPR screens.
DR ChiTaRS; Rft1; mouse.
DR PRO; PR:Q8C3B8; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8C3B8; protein.
DR Bgee; ENSMUSG00000052395; Expressed in proximal tubule and 63 other tissues.
DR ExpressionAtlas; Q8C3B8; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0034203; P:glycolipid translocation; IBA:GO_Central.
DR InterPro; IPR007594; RFT1.
DR PANTHER; PTHR13117; PTHR13117; 1.
DR Pfam; PF04506; Rft-1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..541
FT /note="Protein RFT1 homolog"
FT /id="PRO_0000311287"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 187..220
FT /note="LLYTTVLVLCYAIYLIQLLRSPESAKQLTLPVSR -> VRVVALLFTRLSSL
FT LVPTSMCLCSQYSCCGIVGN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029509"
FT VAR_SEQ 221..541
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029510"
SQ SEQUENCE 541 AA; 60303 MW; EAD30B02F74CF279 CRC64;
MGSQEVLGQA ARLASSGLLL QVLFRLITFV LNAFILRFLS KEIVGIVNVR LTLLYSTTTF
LAREAFRRAC LSGGAQRDWS QTLNLLWLTV PLGIFWSSCL GWVWLQLLEV PDPDVVPYYG
TGVLFFGLSA VVELLGEPFW VLAQAHMFVK LKVLAESMSV ILRSVLTALL VLWLPHWGLY
IFSLAQLLYT TVLVLCYAIY LIQLLRSPES AKQLTLPVSR VTQLLPSISR SRAFVNWKEA
GLAWSFFKQS FLKQILTEGE RYVMTFLNVL NFGDQGVYDI VNNLGSLVAR LIFQPVEESF
YLFFAKVLER EKDASLQKQD DVAVAAAVLE SLLKLALLTG LTMTVFGFAY SQLALDIYGG
AMLSSGSGPV LMRCYCLYVL LLAINGVTEC FMFAAMSKEE VDRYNFTMLA LSSSFLVLSY
LLTSWCGSVG FIMANCFNMG IRITQSLSFI HHYFRESPHR PLAGLRLSPV LLGVFILSAG
ITSVSEAFLC CERGWPARLA HIAVGTICLG VTLGTAFLTE TKLIHFLRTQ LGRSRLSDKM
T
