RFT1_YEAST
ID RFT1_YEAST Reviewed; 574 AA.
AC P38206; D6VPY0; Q05814;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Oligosaccharide translocation protein RFT1;
DE AltName: Full=Requiring fifty-three protein 1;
GN Name=RFT1; OrderedLocusNames=YBL020W; ORFNames=YBL0442;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YMW2;
RX PubMed=7673248; DOI=10.1074/jbc.270.38.22556;
RA Koerte A., Chong T., Li X., Wahane K., Cai M.;
RT "Suppression of the yeast mutation rft1-1 by human p53.";
RL J. Biol. Chem. 270:22556-22564(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-428.
RX PubMed=2832732; DOI=10.1128/mcb.8.2.655-663.1988;
RA Hahn S., Pinkham J., Wei R., Miller R., Guarente L.;
RT "The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent
RT overlapping transcripts.";
RL Mol. Cell. Biol. 8:655-663(1988).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11807558; DOI=10.1038/415447a;
RA Helenius J., Ng D.T.W., Marolda C.L., Walter P., Valvano M.A., Aebi M.;
RT "Translocation of lipid-linked oligosaccharides across the ER membrane
RT requires Rft1 protein.";
RL Nature 415:447-450(2002).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in N-linked oligosaccharide assembly. Required for
CC the translocation of Man(5)GlcNAc(2)-PP-dolichol from the cytoplasmic
CC side to the lumenal side of the endoplasmic reticulum membrane.
CC {ECO:0000269|PubMed:11807558}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:11807558}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11807558}.
CC -!- SIMILARITY: Belongs to the RFT1 family. {ECO:0000305}.
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DR EMBL; U15087; AAA86312.1; -; Genomic_DNA.
DR EMBL; Z35781; CAA84839.1; -; Genomic_DNA.
DR EMBL; M20318; AAA53537.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07100.1; -; Genomic_DNA.
DR PIR; S45754; S45754.
DR RefSeq; NP_009533.1; NM_001178260.1.
DR AlphaFoldDB; P38206; -.
DR SMR; P38206; -.
DR BioGRID; 32678; 535.
DR DIP; DIP-3885N; -.
DR IntAct; P38206; 5.
DR MINT; P38206; -.
DR STRING; 4932.YBL020W; -.
DR TCDB; 2.A.66.3.1; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR iPTMnet; P38206; -.
DR MaxQB; P38206; -.
DR PaxDb; P38206; -.
DR PRIDE; P38206; -.
DR EnsemblFungi; YBL020W_mRNA; YBL020W; YBL020W.
DR GeneID; 852261; -.
DR KEGG; sce:YBL020W; -.
DR SGD; S000000116; RFT1.
DR VEuPathDB; FungiDB:YBL020W; -.
DR eggNOG; KOG2864; Eukaryota.
DR GeneTree; ENSGT00390000011390; -.
DR HOGENOM; CLU_023360_3_0_1; -.
DR InParanoid; P38206; -.
DR OMA; WPGKLFG; -.
DR BioCyc; MetaCyc:G3O-28923-MON; -.
DR BioCyc; YEAST:G3O-28923-MON; -.
DR PRO; PR:P38206; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38206; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IMP:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0034202; F:glycolipid floppase activity; TAS:Reactome.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0034203; P:glycolipid translocation; IMP:SGD.
DR InterPro; IPR007594; RFT1.
DR PANTHER; PTHR13117; PTHR13117; 1.
DR Pfam; PF04506; Rft-1; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..574
FT /note="Oligosaccharide translocation protein RFT1"
FT /id="PRO_0000212420"
FT TOPO_DOM 1..24
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..48
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..110
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..181
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..319
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..413
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..469
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..509
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 510..530
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 531..532
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 554..574
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 74..75
FT /note="IR -> MF (in Ref. 4; AAA53537)"
FT /evidence="ECO:0000305"
FT CONFLICT 207..216
FT /note="PMGVVTSDID -> QWGLSHRTLT (in Ref. 4; AAA53537)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 574 AA; 66211 MW; D619A5362FB65509 CRC64;
MAKKNSQLPS TSEQILERST TGATFLMMGQ LFTKLVTFIL NNLLIRFLSP RIFGITAFLE
FIQGTVLFFS RDAIRLSTLR ISDSGNGIID DDDEEEYQET HYKSKVLQTA VNFAYIPFWI
GFPLSIGLIA WQYRNINAYF ITLPFFRWSI FLIWLSIIVE LLSEPFFIVN QFMLNYAARS
RFESIAVTTG CIVNFIVVYA VQQSRYPMGV VTSDIDKEGI AILAFALGKL AHSITLLACY
YWDYLKNFKP KKLFSTRLTK IKTRENNELK KGYPKSTSYF FQNDILQHFK KVYFQLCFKH
LLTEGDKLII NSLCTVEEQG IYALLSNYGS LLTRLLFAPI EESLRLFLAR LLSSHNPKNL
KLSIEVLVNL TRFYIYLSLM IIVFGPANSS FLLQFLIGSK WSTTSVLDTI RVYCFYIPFL
SLNGIFEAFF QSVATGDQIL KHSYFMMAFS GIFLLNSWLL IEKLKLSIEG LILSNIINMV
LRILYCGVFL NKFHRELFTD SSFFFNFKDF KTVIIAGSTI CLLDWWFIGY VKNLQQFVVN
VLFAMGLLAL ILVKERQTIQ SFINKRAVSN SKDV
