ID   RFU1_ASHGO              Reviewed;         193 AA.
AC   Q75CZ0;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Regulator of free ubiquitin chains 1;
GN   Name=RFU1; OrderedLocusNames=ABR232C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 122; 148-149; 156-157 AND
RP   C-TERMINUS.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Inhibitor of the DOA4 deubiquitinase involved in the
CC       regulation of protein degradation by the proteasome and maintenance of
CC       a normal level of free ubiquitin. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RFU1 family. {ECO:0000305}.
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DR   EMBL; AE016815; AAS51005.2; -; Genomic_DNA.
DR   RefSeq; NP_983181.2; NM_208534.2.
DR   AlphaFoldDB; Q75CZ0; -.
DR   SMR; Q75CZ0; -.
DR   STRING; 33169.AAS51005; -.
DR   PRIDE; Q75CZ0; -.
DR   EnsemblFungi; AAS51005; AAS51005; AGOS_ABR232C.
DR   GeneID; 4619291; -.
DR   KEGG; ago:AGOS_ABR232C; -.
DR   eggNOG; ENOG502S3ZX; Eukaryota.
DR   HOGENOM; CLU_1348926_0_0_1; -.
DR   InParanoid; Q75CZ0; -.
DR   OMA; LKTCVNI; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0061578; F:Lys63-specific deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0070536; P:protein K63-linked deubiquitination; IBA:GO_Central.
DR   GO; GO:0010992; P:ubiquitin recycling; IEA:EnsemblFungi.
PE   3: Inferred from homology;
KW   Endosome; Protease inhibitor; Reference proteome; Thiol protease inhibitor.
FT   CHAIN           1..193
FT                   /note="Regulator of free ubiquitin chains 1"
FT                   /id="PRO_0000376812"
SQ   SEQUENCE   193 AA;  21865 MW;  456C7F45352FDF0E CRC64;
     MKSSAQLCME ARNYNFNAAA PLRLYLATCV RLVEEAQAAA QADDVARAYM LYVRYLDLCM
     HQLSGHREVQ QPVTDAERLS RDEYEQLLRL EVPAVLRLTE ELKSAVDLRH ERGRASLARS
     VVPEAGRGEH SRQEVQLPPS FDEERFNRTV QWFLAAGRSM SLPVSAEEPA VREVFSYPEL
     PKLSMAAESW APS