ID   RFU1_KLULA              Reviewed;         189 AA.
AC   Q6CRY0;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Regulator of free ubiquitin chains 1;
GN   Name=RFU1; OrderedLocusNames=KLLA0D05577g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Inhibitor of the DOA4 deubiquitinase involved in the
CC       regulation of protein degradation by the proteasome and maintenance of
CC       a normal level of free ubiquitin. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RFU1 family. {ECO:0000305}.
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DR   EMBL; CR382124; CAH00405.1; -; Genomic_DNA.
DR   RefSeq; XP_453309.1; XM_453309.1.
DR   AlphaFoldDB; Q6CRY0; -.
DR   SMR; Q6CRY0; -.
DR   STRING; 28985.XP_453309.1; -.
DR   EnsemblFungi; CAH00405; CAH00405; KLLA0_D05577g.
DR   GeneID; 2893240; -.
DR   KEGG; kla:KLLA0_D05577g; -.
DR   eggNOG; ENOG502S3ZX; Eukaryota.
DR   HOGENOM; CLU_1348926_0_0_1; -.
DR   InParanoid; Q6CRY0; -.
DR   OMA; LKTCVNI; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0016579; P:protein deubiquitination; IEA:UniProt.
DR   GO; GO:0010992; P:ubiquitin recycling; IEA:EnsemblFungi.
DR   InterPro; IPR015063; USP8_dimer.
DR   Pfam; PF08969; USP8_dimer; 1.
PE   3: Inferred from homology;
KW   Endosome; Protease inhibitor; Reference proteome; Thiol protease inhibitor.
FT   CHAIN           1..189
FT                   /note="Regulator of free ubiquitin chains 1"
FT                   /id="PRO_0000376814"
SQ   SEQUENCE   189 AA;  21733 MW;  F36F1B1D7F4CA677 CRC64;
     MKSSSHLKQE ALNYRFNSNV PLKLYLTTCI SIVEEAQNSA RCNNMERAYL LYIRYLDLCL
     NRLVHHSEVN TSVESLYKRE YFQLLKLEVP AIMKITEELK IKIDARYKSL ANNVATSTYV
     RSPAVMTTNP SDEINLPSSF DEQKFNQSIS WFNHSSKHFS AQSDSNNEYP ELPELNATST
     SVTYTMPAL