ID   RFU1_VANPO              Reviewed;         197 AA.
AC   A7TJP2;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Regulator of free ubiquitin chains 1;
GN   Name=RFU1; ORFNames=Kpol_1058p8;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: Inhibitor of the DOA4 deubiquitinase involved in the
CC       regulation of protein degradation by the proteasome and maintenance of
CC       a normal level of free ubiquitin. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RFU1 family. {ECO:0000305}.
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DR   EMBL; DS480403; EDO17471.1; -; Genomic_DNA.
DR   RefSeq; XP_001645329.1; XM_001645279.1.
DR   AlphaFoldDB; A7TJP2; -.
DR   SMR; A7TJP2; -.
DR   STRING; 436907.A7TJP2; -.
DR   EnsemblFungi; EDO17471; EDO17471; Kpol_1058p8.
DR   GeneID; 5545689; -.
DR   KEGG; vpo:Kpol_1058p8; -.
DR   eggNOG; ENOG502S3ZX; Eukaryota.
DR   HOGENOM; CLU_1348926_0_0_1; -.
DR   InParanoid; A7TJP2; -.
DR   OMA; LKTCVNI; -.
DR   OrthoDB; 1368651at2759; -.
DR   PhylomeDB; A7TJP2; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0016579; P:protein deubiquitination; IEA:UniProt.
DR   InterPro; IPR015063; USP8_dimer.
DR   Pfam; PF08969; USP8_dimer; 1.
PE   3: Inferred from homology;
KW   Endosome; Protease inhibitor; Reference proteome; Thiol protease inhibitor.
FT   CHAIN           1..197
FT                   /note="Regulator of free ubiquitin chains 1"
FT                   /id="PRO_0000376817"
SQ   SEQUENCE   197 AA;  22865 MW;  4C52E3FC843A60BF CRC64;
     MFIGMKSSSQ LRQEALEYTY NANTPLKIYL KVCVSILDKA QLEFQKGDIF SAYVLYYRYA
     DLIANKLSDH SELALVAERD QTVLHREEYY QLVKLELPAV LKIVEDLQKN IDLDYNKIQL
     SLSKNIAKQQ KKPNHVSEPV LLPKTFNENR FNQSISFFNN MNSNITLENK MHGNNNNNTH
     QELLYPELPT LSNASYI