ID   RFU1_YEAS7              Reviewed;         200 AA.
AC   A7A0X8;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Regulator of free ubiquitin chains 1;
GN   Name=RFU1; ORFNames=SCY_3652;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Inhibitor of the DOA4 deubiquitinase involved in the
CC       regulation of protein degradation by the proteasome and maintenance of
CC       a normal level of free ubiquitin. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with BRO1 and DOA4. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RFU1 family. {ECO:0000305}.
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DR   EMBL; AAFW02000167; EDN59619.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7A0X8; -.
DR   SMR; A7A0X8; -.
DR   EnsemblFungi; EDN59619; EDN59619; SCY_3652.
DR   HOGENOM; CLU_1348926_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Endosome; Protease inhibitor; Thiol protease inhibitor.
FT   CHAIN           1..200
FT                   /note="Regulator of free ubiquitin chains 1"
FT                   /id="PRO_0000376816"
SQ   SEQUENCE   200 AA;  22850 MW;  1C93378ED78C41A4 CRC64;
     MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI YYFRYVDLLT
     NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC KIIESLRTQI DSQYSKLQTS
     LANNIAKPNI NANTTPVQVE QQPLPKKSFD EYSFNQSISF FQKISNAQLN TGASSQSQAT
     ARDEAYRLNY PELPRLTFST