RFX1_MOUSE
ID RFX1_MOUSE Reviewed; 963 AA.
AC P48377; Q6PGH8;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=MHC class II regulatory factor RFX1;
DE AltName: Full=Regulatory factor X 1;
DE AltName: Full=Transcription factor RFX1;
GN Name=Rfx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Spleen;
RX PubMed=8289803; DOI=10.1128/mcb.14.2.1230-1244.1994;
RA Reith W., Ucla C., Barras E., Gaud A., Durand B., Herrero-Sanchez C.,
RA Kobr M., Mach B.;
RT "RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel
RT family of homodimeric and heterodimeric DNA-binding proteins.";
RL Mol. Cell. Biol. 14:1230-1244(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBUNIT.
RX PubMed=15229132; DOI=10.1095/biolreprod.104.032268;
RA Horvath G.C., Kistler W.S., Kistler M.K.;
RT "RFX2 is a potential transcriptional regulatory factor for histone H1t and
RT other genes expressed during the meiotic phase of spermatogenesis.";
RL Biol. Reprod. 71:1551-1559(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulatory factor essential for MHC class II genes
CC expression. Binds to the X boxes of MHC class II genes (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer; binds DNA as a homodimer (By similarity).
CC Heterodimer; heterodimerizes with RFX2 and RFX3 (PubMed:15229132).
CC {ECO:0000250|UniProtKB:P22670, ECO:0000269|PubMed:15229132}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00858}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; X76088; CAA53702.1; -; mRNA.
DR EMBL; CH466525; EDL10930.1; -; Genomic_DNA.
DR EMBL; BC057018; AAH57018.1; -; mRNA.
DR CCDS; CCDS22468.1; -.
DR PIR; A55926; A55926.
DR RefSeq; NP_033081.3; NM_009055.4.
DR AlphaFoldDB; P48377; -.
DR SMR; P48377; -.
DR BioGRID; 202872; 3.
DR STRING; 10090.ENSMUSP00000005600; -.
DR iPTMnet; P48377; -.
DR PhosphoSitePlus; P48377; -.
DR SwissPalm; P48377; -.
DR EPD; P48377; -.
DR jPOST; P48377; -.
DR MaxQB; P48377; -.
DR PaxDb; P48377; -.
DR PeptideAtlas; P48377; -.
DR PRIDE; P48377; -.
DR ProteomicsDB; 255188; -.
DR Antibodypedia; 26599; 185 antibodies from 25 providers.
DR DNASU; 19724; -.
DR Ensembl; ENSMUST00000005600; ENSMUSP00000005600; ENSMUSG00000031706.
DR GeneID; 19724; -.
DR KEGG; mmu:19724; -.
DR UCSC; uc009mlv.2; mouse.
DR CTD; 5989; -.
DR MGI; MGI:105982; Rfx1.
DR VEuPathDB; HostDB:ENSMUSG00000031706; -.
DR eggNOG; KOG3712; Eukaryota.
DR GeneTree; ENSGT01050000244879; -.
DR HOGENOM; CLU_010393_1_0_1; -.
DR InParanoid; P48377; -.
DR OMA; QVHSVQA; -.
DR OrthoDB; 346630at2759; -.
DR PhylomeDB; P48377; -.
DR TreeFam; TF321340; -.
DR BioGRID-ORCS; 19724; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Rfx1; mouse.
DR PRO; PR:P48377; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P48377; protein.
DR Bgee; ENSMUSG00000031706; Expressed in ascending aorta and 217 other tissues.
DR ExpressionAtlas; P48377; baseline and differential.
DR Genevisible; P48377; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR039779; RFX-like.
DR InterPro; IPR007668; RFX1_trans_act.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12619; PTHR12619; 1.
DR Pfam; PF04589; RFX1_trans_act; 1.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..963
FT /note="MHC class II regulatory factor RFX1"
FT /id="PRO_0000215287"
FT DNA_BIND 423..498
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 1..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 174..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 899..948
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..43
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 905..922
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 54
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P22670"
FT MOD_RES 962
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P22670"
FT MOD_RES 963
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P22670"
FT CONFLICT 169..170
FT /note="QH -> HD (in Ref. 1; CAA53702)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 963 AA; 103707 MW; C868182B206BA9F0 CRC64;
MATQSYVTEL QAAPQASQPP QAPPQALPQP PPPAAPQPPA AATPQPQYVT ELQSPQPQTQ
PPGSQKQYVA ELPAAPAPSQ PATPAPSPVA QQYIVVTVSE GAMRASETVS EASPSSTASQ
TGVPTQVVQQ VQGTQQRLLV QASVQAKPGH VSPLQLTNIQ VPQQAIPTQH LVVQSPAPGT
KSGQVSLTVH SAQQVHSAPE RSPVQANNST SKTAGTPAAT VQQLQVHSVQ QSVPVTQERS
VVQATPQTKA GPVQQLTVQG LQPVHVAQEV QQLPQVPVPH VYSSQVQYVE GGDASYTASA
IRSSTYQYPE TPIYTQTAGT SYYEASGTAA QVSTPATSQT VASSGSVPMY VSGSPIVASS
SSSEAGASNS SVGAGGNGGG GSSGGGSGGS SGSGAGTYVI QGGYMLGNAS QSYSHTTRAS
PATVQWLLDN YETAEGVSLP RSTLYCHYLL HCQEQKLEPV NAASFGKLIR SVFMGLRTRR
LGTRGNSKYH YYGLRIKASS PLLRLMEDQQ HMAMRGQPFS QKQRLKPIQK MEGVANGVAV
GQQSTGLSDI SAQVQQYQQF LDASRSLPDF AELDLQGKVL PEGVGPGDIK AFQVLYREHC
EAIVDVMVNL QFTLVETLWK TFWRYNLSQP SEAPPLAVHD EAEKRLPRAS LVLLSKFQPV
LQWTKHCDNV LYQGLVEILI PDVLRPIPSA LTQAIRNFAK SLESWLTHAM VNIPEEMLRV
KVAAAGAFAQ TLRRYTSLNH LAQAARAVLQ NTAQINQMLS DLNRVDFANV QEQASWVCRC
EDRVVQRLEQ DFKVTLQQQN SLEQWAAWLD GVVSQVLKPY QGSSGFPKAA KLFLLKWSFY
SSMVIRDLTL RSAASFGSFH LIRLLYDEYM YYLIEHRVAQ AKGETPIAVM GEFANLATSL
NPLDPDKDEE EEEEEESEDE LPQDISLAAG SESPALGPEA LEPPAKLART DTRGLFVQAL
PSS
