RFX1_YEAST
ID RFX1_YEAST Reviewed; 811 AA.
AC P48743; D6VYI0;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=RFX-like DNA-binding protein RFX1;
GN Name=RFX1; Synonyms=CRT1; OrderedLocusNames=YLR176C; ORFNames=L9470.18;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- INTERACTION:
CC P48743; P14922: CYC8; NbExp=2; IntAct=EBI-15036, EBI-18215;
CC P48743; P16649: TUP1; NbExp=2; IntAct=EBI-15036, EBI-19654;
CC -!- MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; U17246; AAB67470.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09496.1; -; Genomic_DNA.
DR PIR; S51421; S51421.
DR RefSeq; NP_013277.1; NM_001182063.1.
DR AlphaFoldDB; P48743; -.
DR SMR; P48743; -.
DR BioGRID; 31447; 176.
DR DIP; DIP-6790N; -.
DR IntAct; P48743; 18.
DR MINT; P48743; -.
DR STRING; 4932.YLR176C; -.
DR iPTMnet; P48743; -.
DR MaxQB; P48743; -.
DR PaxDb; P48743; -.
DR PRIDE; P48743; -.
DR EnsemblFungi; YLR176C_mRNA; YLR176C; YLR176C.
DR GeneID; 850873; -.
DR KEGG; sce:YLR176C; -.
DR SGD; S000004166; RFX1.
DR VEuPathDB; FungiDB:YLR176C; -.
DR eggNOG; KOG3712; Eukaryota.
DR GeneTree; ENSGT01050000244970; -.
DR HOGENOM; CLU_011526_2_0_1; -.
DR InParanoid; P48743; -.
DR OMA; AQYASSC; -.
DR BioCyc; YEAST:G3O-32301-MON; -.
DR PRO; PR:P48743; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P48743; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR039779; RFX-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12619; PTHR12619; 1.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..811
FT /note="RFX-like DNA-binding protein RFX1"
FT /id="PRO_0000215295"
FT DNA_BIND 285..360
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 48..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 377..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..432
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 173
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 811 AA; 90584 MW; 116A88B7DDE4FBF0 CRC64;
MVIFKERKPT ENLFTRKIPA KYFIFSPSFL SVHYFEFYLP MSGDNNIEPT SRGSNDNSNG
PSNGSSVNSN RYSLNAPKYS SQPPPASHTY LPPMSVNIPP IASKSSSIYS LLHQSSPRPE
TPNPILPPLI GSGPGSHKPS PTPTQPPAQP ATQRQPATYS VYPASISLNR SNSSAYPLSF
KSEETLNNNP PTAAKRTNTF PSIPSSTKKQ KTSQEKRISS ISRRNTQEII AKQIAENNKS
KTIEEYAQIV KHAEIKVLSM DSQNTSKAAL QLAEQNRERE RQVFALLWLM KNCKSQHDSY
VPRGKIFAQY ASSCSQNNLK PLSQASLGKL IRTVFPDLTT RRLGMRGQSK YHYCGLKLTV
NESGSVSLNN NNASLSLVHN NDPISPLSSP SPSSPSPQVP NVSSPFSLNR KSLSRTGSPV
KQSSNDNPNE PELESQHPNE TEANKLDSLP PAANNPTGTL SSDELTFTHD LIEKVFNCND
KLSDNYNTQI LSNTEHPLLT SYKLDFPKIP AGVLPTDTDS DVISSLESLY HIHCNSVYEC
IKFLKSDNIS NALFFSNSNS ISPTMFNLFI SEPLIDWVTK CDLITYTGLI KFFSQFIIHS
NEISDSIIQK LESMIKLLPE QINKAVLELP KALVQRKLSI INNFTKLVKK LIKLLKFILN
FLKSFPIFKS GMNNDWKNIV NLDDILEMMI NEDDTNSETN TIMQHLQGFC QVFVTKFLNS
SMSVSNDPSV SIECKSLNEM IKDFCSFISL QSKFSCLKLI DCSTRFRNAI IGDISLKSNE
NLLSWLFLNN VMGQLLNYCF EVMKFVNGLK V
