ID   RFX2_BOVIN              Reviewed;         707 AA.
AC   A6QLW9;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=DNA-binding protein RFX2;
DE   AltName: Full=Regulatory factor X 2;
GN   Name=RFX2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal medulla;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that acts as a key regulator of
CC       spermatogenesis. Acts by regulating expression of genes required for
CC       the haploid phase during spermiogenesis, such as genes required for
CC       cilium assembly and function. Recognizes and binds the X-box, a
CC       regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on
CC       promoters. Probably activates transcription of the testis-specific
CC       histone gene H1-6. {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBUNIT: Homodimer; probably only forms homodimers in testis.
CC       Heterodimer; heterodimerizes with RFX1 and RFX3.
CC       {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B2GV50,
CC       ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm
CC       {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus
CC       and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; BC148113; AAI48114.1; -; mRNA.
DR   RefSeq; NP_001095642.1; NM_001102172.1.
DR   AlphaFoldDB; A6QLW9; -.
DR   SMR; A6QLW9; -.
DR   STRING; 9913.ENSBTAP00000023492; -.
DR   PaxDb; A6QLW9; -.
DR   PRIDE; A6QLW9; -.
DR   Ensembl; ENSBTAT00000023492; ENSBTAP00000023492; ENSBTAG00000017661.
DR   GeneID; 534475; -.
DR   KEGG; bta:534475; -.
DR   CTD; 5990; -.
DR   VEuPathDB; HostDB:ENSBTAG00000017661; -.
DR   VGNC; VGNC:33894; RFX2.
DR   eggNOG; KOG3712; Eukaryota.
DR   GeneTree; ENSGT01050000244879; -.
DR   HOGENOM; CLU_010393_1_1_1; -.
DR   InParanoid; A6QLW9; -.
DR   OrthoDB; 346630at2759; -.
DR   TreeFam; TF321340; -.
DR   Proteomes; UP000009136; Chromosome 7.
DR   Bgee; ENSBTAG00000017661; Expressed in spermatid and 101 other tissues.
DR   ExpressionAtlas; A6QLW9; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0001675; P:acrosome assembly; ISS:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0007286; P:spermatid development; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR007668; RFX1_trans_act.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 1.
DR   Pfam; PF04589; RFX1_trans_act; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; Differentiation; DNA-binding;
KW   Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..707
FT                   /note="DNA-binding protein RFX2"
FT                   /id="PRO_0000380693"
FT   DNA_BIND        204..279
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          297..337
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        313..337
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         33
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48378"
FT   MOD_RES         420
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:B2GV50"
SQ   SEQUENCE   707 AA;  77673 MW;  8005833EA16721E1 CRC64;
     MQNSESGSDS PASVALCSSA AAQAPVAQPV TASPQRVLVQ AAGSAPKGAQ MQPLSLPRVQ
     QVTQQVQPAQ HVYPAQLQYV EGGDAVFTNG ALRTAYAYNP EPQMYAPSSA ASYFEAPGGA
     QVTVAGSSPP AVPSHSMVGI TMDVGGSPIV SSAGAYLIHG GMDGTRHTLA HTSRSSPATL
     EMAIENLQKS EGITSHKSGL LNSHLQWLLD NYETAEGVSL PRSSLYNHYL RHCQEHKLDP
     VNAASFGKLI RSVFMGLRTR RLGTRGNSKY HYYGIRLKPD SPLNRLQEDT QYMAMRQQPV
     HQKPRYRPAQ KTDSLGESGS HSSLHSTPEQ AMAAQSQHHQ QYIDVSHVFP EFPAPDLGSV
     LLQESITLHD VKALQLAYRR HCEATLDVVM NLQFHYIEKL WLSFWNSKSS SDGPTSLPAS
     DEEPEGAVLP KDKLVSLCKC DPVLRWMRTC DHILYQALVE ILIPDVLRPV PSTLTQAIRN
     FAKSLEGWLT NAMSDFPQQV IQTKVGVVSA FAQTLRRYTS LNHLAQAARA VLQNTSQINQ
     MLSDLNRVDF ANVQEQASWV CQCEEGVVQR LEQDFKLTLQ QQSSLDQWAS WLDNVVTQVL
     KQHAGSPSFP KAARQFLLKW SFYSSMVIRD LTLRSAASFG SFHLIRLLYD EYMFYLVEHR
     VAEATGETPI AVMGEFNDLA SLSLTLLDKG GCVWALPPVH SGGGGSP